Anti-osteoporotic activity of puerarin 6'-O-xyloside on ovariectomized mice and its potential mechanism

Autor: Bingpu Chen, Guangfu Pang, Hai-ling Huang, Ji-sheng Xie, Jianhai Chen, Hai Li
Rok vydání: 2015
Předmět:
Pueraria
Time Factors
Osteoporosis
Pharmaceutical Science
chemistry.chemical_compound
0302 clinical medicine
Puerarin
Drug Discovery
Glycosides
Puerarin 6'-O-xyloside
Cells
Cultured
Osteoporosis
Postmenopausal
Mice
Inbred ICR
biology
Bone Density Conservation Agents
Phosphorus
General Medicine
Blot
030220 oncology & carcinogenesis
Ovariectomized rat
Molecular Medicine
Alkaline phosphatase
Female
Bone Remodeling
medicine.medical_specialty
Ovariectomy
Bone and Bones
03 medical and health sciences
Osteoprotegerin
Internal medicine
medicine
Animals
Humans
Cell Proliferation
Pharmacology
Osteoblasts
Dose-Response Relationship
Drug
business.industry
RANK Ligand
medicine.disease
biology.organism_classification
Alkaline Phosphatase
Isoflavones
Disease Models
Animal
Endocrinology
Complementary and alternative medicine
chemistry
Calcium
business
030217 neurology & neurosurgery
Biomarkers
Zdroj: Pharmaceutical biology. 54(1)
ISSN: 1744-5116
Popis: Osteoporosis is one of the most common bone diseases, and radix of Pueraria lobata (Willd.) Ohwi possesses an obvious therapeutical effect on postmenopausal osteoporosis.This study investigates the anti-osteoporotic activity of the puerarin 6"-O-xyloside (PXY) on ovariectomized mice and its related mechanism.Osteoporotic mice model was established by ovariectomy (OVX). A total of 50 mice were divided into five groups (n = 10): sham, OVX group, PXY treatment groups (20, 40, and 60 mg/kg/d, i.p.). After 12 weeks' treatment, body weights were recorded. Then, mice were sacrificed, and serum samples were collected to determine the blood calcium, blood phosphorus, alkaline phosphatase (ALP), and osteoprotegerin (OPG) concentrations and uterine index was assayed. The thigh-bones of mice were collected to evaluate histopathological changes. In the in vitro experiment, the effect of PXY on osteoblasts' proliferation was evaluated and western blotting was performed to determine expressions of OPG and the receptor activators of NF-κB ligand (RANKL), as well as the ratio of OPG/RANKL.PXY (40 and 60 mg/kg/d, i.p.) obviously decreased body weights and increased uterine index of OVX (p0.05), and improved osteoporotic syndromes of OVX mice; PXY also significantly increased the concentrations of blood calcium, blood phosphorus, ALP, and OPG of OVX mice (p0.05); moreover, PXY obviously up-regulated the ratio of OPG/RANKL (p0.05).Our results demonstrated that the puerarin 6"-O-xyloside possesses significant anti-osteoporotic activity on ovariectomy mice.
Databáze: OpenAIRE
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