Cyclin C influences the timing of mitosis in fission yeast
| Autor: | Zsolt Szilagyi, Vera Baraznenok, Gabor Banyai, Claes M. Gustafsson, Olga Khorosjutina |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Cell cycle checkpoint Cyclin A Mitosis S Phase 03 medical and health sciences Mediator Cyclin C Cyclin-dependent kinase Cyclins Gene Expression Regulation Fungal Schizosaccharomyces Phosphorylation Molecular Biology Mediator Complex biology Cell Cycle Cell Cycle Checkpoints Articles Cell Biology Cell cycle Cyclin-Dependent Kinase 8 Cyclin-Dependent Kinases 3. Good health Cell biology 030104 developmental biology biology.protein Cyclin-dependent kinase 8 RNA Polymerase II Cell Division Cyclin A2 |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e16-11-0787 |
| Popis: | A number of genes are periodically transcribed during cell cycle progression. How the periodic transcription patterns of these genes are achieved is not completely understood. Cyclin C, a component of Mediator, plays an essential role in periodic transcription and the timing of cell cycle progression. The multiprotein Mediator complex is required for the regulated transcription of nearly all RNA polymerase II–dependent genes. Mediator contains the Cdk8 regulatory subcomplex, which directs periodic transcription and influences cell cycle progression in fission yeast. Here we investigate the role of CycC, the cognate cyclin partner of Cdk8, in cell cycle control. Previous reports suggested that CycC interacts with other cellular Cdks, but a fusion of CycC to Cdk8 reported here did not cause any obvious cell cycle phenotypes. We find that Cdk8 and CycC interactions are stabilized within the Mediator complex and the activity of Cdk8-CycC is regulated by other Mediator components. Analysis of a mutant yeast strain reveals that CycC, together with Cdk8, primarily affects M-phase progression but mutations that release Cdk8 from CycC control also affect timing of entry into S phase. |
| Databáze: | OpenAIRE |
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