A simple method for studying steroid-antibody interaction under conditions of thermodynamic equilibrium
| Autor: | Harvey S. Schiller |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Thermodynamic equilibrium
medicine.medical_treatment Biophysics Antigen-Antibody Complex Polyethylene glycol Biochemistry Antibodies Steroid Antigen-Antibody Reactions chemistry.chemical_compound Methods medicine Animals Equilibrium dialysis Molecular Biology Progesterone Equilibrium constant Scatchard plot Sheep Chromatography Estriol Chemistry Cell Biology Kinetics Thermodynamics Rabbits Dialysis |
| Zdroj: | Analytical Biochemistry. 76:142-148 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(76)90272-4 |
| Popis: | We compare the classic method of equilibrium dialysis for achieving thermodynamic equilibrium with a method in which polyethylene glycol is used to separate antibody-bound and unbound steroid. Antisera to both polar and nonpolar steroids were studied. Comparable Scatchard plots were obtained by the two methods. Equilibrium constants of association of 10 9 to 10 10 liters/mol of the different antisera were determined by both methods. The described method should simplify the study of steroid-antibody interaction. In addition, this method will permit investigators to characterize quickly and conveniently the antisera to be used for sensitive radioimmunoassays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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