Expression profiling of the wheat pathogen Zymoseptoria tritici reveals genomic patterns of transcription and host-specific regulatory programs
| Autor: | Rachel B. Brem, Amitava Bhattacharyya, Stephan Poppe, Tiffany Hsu, Ronny Kellner, Eva H. Stukenbrock |
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| Rok vydání: | 2014 |
| Předmět: |
Virulence
Genome Vaccine Related Ascomycota Phylogenetics Gene Expression Regulation Fungal Genetics 2.2 Factors relating to the physical environment Gene Ecology Evolution Behavior and Systematics Triticum Phylogeny Plant Diseases Evolutionary Biology biology Prevention supernumerary chromosomes gene duplication food and beverages host adaptation biology.organism_classification Pathogenicity island Infectious Diseases Fungal Gene Expression Regulation Mycosphaerella graminicola plant pathogenic fungi Host-Pathogen Interactions Brachypodium distachyon Host adaptation Biochemistry and Cell Biology RNA-seq Infection Transcriptome Biotechnology Research Article Developmental Biology |
| Zdroj: | Genome biology and evolution, vol 6, iss 6 Genome Biology and Evolution |
| Popis: | Host specialization by pathogens requires a repertoire of virulence factors as well as fine-tuned regulation of gene expression. The fungal wheat pathogen Zymoseptoria tritici (synonym Mycosphaerella graminicola) is a powerful model system for the discovery of genetic elements that underlie virulence and host specialization. We transcriptionally profiled the early stages of Z. tritici infection of a compatible host (wheat) and a noncompatible host (Brachypodium distachyon). The results revealed infection regulatory programs common to both hosts and genes with striking wheat-specific expression, with many of the latter showing sequence signatures of positive selection along the Z. tritici lineage. Genes specifically regulated during infection of wheat populated two large clusters of coregulated genes that may represent candidate pathogenicity islands. On evolutionarily labile, repeat-rich accessory chromosomes (ACs), we identified hundreds of highly expressed genes with signatures of evolutionary constraint and putative biological function. Phylogenetic analyses suggested that gene duplication events on these ACs were rare and largely preceded the diversification of Zymoseptoria species. Together, our data highlight the likely relevance for fungal growth and virulence of hundreds of Z. tritici genes, deepening the annotation and functional inference of the genes of this model pathogen. |
| Databáze: | OpenAIRE |
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