Type of Renal Replacement Therapy (Hemodialysis versus Peritoneal Dialysis) Does Not Affect Cytokine Gene Expression or Clinical Parameters of Renal Transplant Candidates
| Autor: | Marian Klinger, Marcelina Żabińska, Agnieszka Lepiesza, Maria Boratyńska, Oktawia Mazanowska, Agnieszka Gomółkiewicz, P. Chudoba, Krzysztof Korta, Dorota Kamińska, Piotr Dziegiel, Mirosław Banasik, Katarzyna Kościelska-Kasprzak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Adolescent Article Subject medicine.medical_treatment lcsh:Medicine General Biochemistry Genetics and Molecular Biology Peritoneal dialysis Proinflammatory cytokine Renal Dialysis Humans Medicine Renal replacement therapy Kidney transplantation Dialysis Aged General Immunology and Microbiology business.industry lcsh:R General Medicine Middle Aged medicine.disease Kidney Transplantation Transplantation Gene Expression Regulation Immunology Cytokines Interleukin 18 Hemodialysis business Peritoneal Dialysis Research Article |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2015/797490 |
| Popis: | Patients with renal failure suffer from immune disturbances, caused by uremic toxins and influenced by dialysis treatment. The aim of the present study was to reveal whether type of dialysis modality (hemodialysis, HD, versus peritoneal dialysis, PD) differentially affects the immunocompetence, particularly the expression of genes involved in the immune response.Material. 87 renal transplant candidates (66 HD, 21 PD) were included in the study.Methods. The peripheral blood RNA samples were obtained with the PAXgene Blood system just before transplantation. The gene expression ofCASP3, FAS, TP53, FOXP3, IFNG, IL2, IL6, IL8, IL10, IL17, IL18, LCN2, TGFB1, andTNFwas assessed with real-time PCR on custom-designed low density arrays (TaqMan). Gene expression data were analyzed in relation to pretransplant clinical parameters.Results. The mean expression of examined genes showed no significant differences between PD and HD with the exception ofFAS, expression of which was 30% higher in PD patients compared to the HD group. There was nonsignificantly higher expression of proinflammatory cytokines in the PD group. The clinical inflammatory parameters (CRP, albumin, cholesterol, and hemoglobin levels) did not differ between the groups.Conclusion. Type of renal replacement therapy exerts no differential effect on cytokine gene expression or inflammatory clinical parameters. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|