Cupric stress induces oxidative damage marked by accumulation of H2O2and changes to chloroplast ultrastructure in primary leaves of beans (Phaseolus vulgarisL.)
| Autor: | Houda Bouazizi, Ezzeddine El Ferjani, Anja Geitmann, Hager Jouili |
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| Rok vydání: | 2010 |
| Předmět: |
Chloroplasts
Antioxidant medicine.medical_treatment Palisade cell Antioxidants General Biochemistry Genetics and Molecular Biology Lipid peroxidation chemistry.chemical_compound Microscopy Electron Transmission Malondialdehyde medicine General Environmental Science Dose-Response Relationship Drug biology Fabaceae Starch Hydrogen Peroxide biology.organism_classification Enzyme assay Plant Leaves Oxidative Stress Neurology Biochemistry chemistry Catalase Seeds biology.protein Lipid Peroxidation Phaseolus Copper Peroxidase |
| Zdroj: | Acta Biologica Hungarica. 61:191-203 |
| ISSN: | 1588-256X 0236-5383 |
| DOI: | 10.1556/abiol.61.2010.2.7 |
| Popis: | The effect of copper excess (CuSO 4 ) on lipid peroxidation, H2O2 content, and antioxidative enzyme activities was studied in primary leaves of bean seedlings. Fourteen-day-old bean seedlings were cultured in a nutrient solution containing Cu 2+ at various concentrations (50 and 75 microM) for 3 days. Excess of copper significantly increased malondialdehyde content and endogenous H2O2 . This radical accumulated in the intercellular spaces of palisade mesophyll cells. In addition, cupric stress induced changes in antioxidant enzyme activities. GPX (guaiacol peroxidase, EC 1.11.1.7) activity was decreased in 50 microM Cu-stressed leaves whereas 75 microM of CuSOP 4 resulted in an increase of enzyme activity. On the contrary, CAT (catalase, EC 1.11.1.6) activity was stimulated at 50 microM CuSO 4 but unaltered at 75 microM CuSO 4 . Transmission electron microscopy revealed that excess copper induced changes in the ultrastructure of chloroplasts visible in form of a deterioration in the grana structure and the accumulation and swelling of starch grains in the stroma. |
| Databáze: | OpenAIRE |
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