Effect of thymoquinone on Fusobacterium nucleatum-associated biofilm and inflammation
| Autor: | Masao Ishikawa, Miad Elahi, Tamiko Nagao, Ayano Tada, Koji Shibuya, Tomomi Kuwahara, Haruyuki Imaohji, Hisashi Yamasaki, Hirofumi Yagi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research dental plaque THP-1 Cells thymoquinone Gingiva Inflammation Dental plaque Biochemistry biofilm Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine black cumin Genetics medicine Benzoquinones Actinomyces Humans Plant Oils Periodontitis Molecular Biology Thymoquinone Microscopy Confocal biology Fusobacterium nucleatum oral care Tumor Necrosis Factor-alpha Biofilm cleansing Articles Fusobacterium medicine.disease biology.organism_classification Thymol 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Biofilms Actinomyces naeslundii Molecular Medicine medicine.symptom |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Periodontitis affects oral tissues and induces systemic inflammation, which increases the risk of cardiovascular disease and metabolic syndrome. Subgingival plaque accumulation is a trigger of periodontitis. Fusobacterium nucleatum (FN) contributes to subgingival biofilm complexity by intercalating with early and late bacterial colonizers on tooth surfaces. In addition, inflammatory responses to FN are associated with the progression of periodontitis. Nigella sativa Lin. seed, which is known as black cumin (BC), has been used as a herbal medicine to treat ailments such as asthma and infectious diseases. The current study examined the inhibitory effect of BC oil and its active constituents, thymol (TM) and thymoquinone (TQ), on FN‑associated biofilm and inflammation. FN‑containing biofilms were prepared by co‑cultivation with an early dental colonizer, Actinomyces naeslundii (AN). The stability and biomass of FN/AN dual species biofilms were significantly higher compared with FN alone. This effect was retained even with prefixed cells, indicating that FN/AN co‑aggregation is mediated by physicochemical interactions with cell surface molecules. FN/AN biofilm formation was significantly inhibited by 0.1% TM or TQ. Confocal laser scanning microscopy indicated that treatment of preformed FN/AN biofilm with 0.01% of BC, TM or TQ significantly reduced biofilm thickness, and TQ demonstrated a cleansing effect equivalent to that of isopropyl methylphenol. TQ dose‑dependently suppressed TNF‑α production from a human monocytic cell line, THP‑1 exposed to FN, yet showed no toxicity to THP‑1 cells. These results indicated that oral hygiene care using TQ could reduce FN‑associated biofilm and inflammation in gingival tissue. |
| Databáze: | OpenAIRE |
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