Xenon pressure dependence on the synchronized burst inhibition of rat cortical neuronal network cultured on multi-electrode arrays
| Autor: | Kenji Yamazaki, Tsutomu Uchida, Daisuke Ito, Ryutaro Tanabe, Koichiro Shimada, Tatsuya Kubota, Kazutoshi Gohara |
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| Rok vydání: | 2017 |
| Předmět: |
Partial pressure
Xenon chemistry.chemical_element General anesthesia Oxygen Multi-electrode array Article lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine 030202 anesthesiology Biological neural network lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Inhibitory effect Cultured neuronal network business.industry General Neuroscience Electrical engineering Pressure dependence Synchronized burst chemistry Electrode Biophysics business 030217 neurology & neurosurgery |
| Zdroj: | IBRO Reports IBRO Reports, Vol 3, Iss C, Pp 45-54 (2017) |
| ISSN: | 2451-8301 |
| Popis: | Mature rat cortical neuronal networks cultured on multi-electrode arrays (MEAs) are known to show spontaneous synchronized bursts accompanied by independent single spikes. The spontaneous synchronized bursts can be inhibited by Xe gas. In this study, we adjust the Xe gas pressure to control the amount of Xe in a neuron-cultured MEA medium. We show that the synchronized bursts cease completely within several minutes by applying Xe gas at partial pressures above 0.3 MPa. After depressurizing and purging with fresh air, the synchronized bursts recover to their original frequency. Thus, we confirmed that Xe acts as a network-activity inhibitor of the cultured neuronal network on MEAs. But below 0.3 MPa, the synchronized bursts are inhibited only partially, depending on the Xe partial pressure. Based on the partial-pressure influence on the change of the neuronal network activities, we find the critical concentration of Xe for the inhibition effect to be approximately 9.5 mM, a value above which more than 90% of the synchronized burst activity is inhibited. Further systematic observations with Xe-air mixed gases show that pressurized air with a small amount of Xe suppresses the inhibition of synchronized bursts, suggesting an air component that can accelerate the synchronized bursts. |
| Databáze: | OpenAIRE |
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