Could formaldehyde induce mutagenic and cytotoxic effects in buccal epithelial cells during anatomy classes?
| Autor: | Ronara-Gerhardt Silva, Letícia-Nogueira da Gama-de-Souza, Haniel-Serpa Nascimento, Karla-Loureiro Almeida-Coburn, Cristiani-Sartorio Menegardo, Diego-Coelho Lorenzoni, Leon-Penido Pinheiro, José Fernando Castanha Henriques, Willian-Grassi Bautz |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Programmed cell death 03 medical and health sciences Fixatives Young Adult 0302 clinical medicine Formaldehyde Medicine Cytotoxic T cell Humans Feulgen stain ALDEÍDOS Cytotoxicity General Dentistry Carcinogen Oral Medicine and Pathology business.industry Cytotoxins Research Mouth Mucosa Epithelial Cells 030206 dentistry Buccal administration Anatomy CIENCIAS MÉDICAS [UNESCO] 030104 developmental biology Otorhinolaryngology Mutagenesis Micronucleus test UNESCO::CIENCIAS MÉDICAS Surgery Female business Micronucleus |
| Zdroj: | Medicina Oral, Patología Oral y Cirugía Bucal Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Lorenzoni, Diego Coelho ; Pinheiro, Leon Penido ; Nascimento, Haniel Serpa ; Menegardo, Cristiani Sartorio ; Silva, Ronara Gerhardt ; Bautz, Willian Grassi ; Henriques, José Fernando ; Almeida Coburn, Karla Loureiro ; da Gama de Souza, Letícia Nogueira. Could formaldehyde induce mutagenic and cytotoxic effects in buccal epithelial cells during anatomy classes?. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 22 1 2017: 7 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname |
| ISSN: | 1698-6946 1698-4447 |
| Popis: | Background Due to increased formaldehyde exposure, carcinogenic to humans, several researches have been studying the potential toxicity and the safe levels for human beings. The aim of this study was to investigate mutagenicity and cytotoxicity in buccal epithelial exfoliated cells (BEC) of students subjected to formaldehyde (FA) during anatomy classes. Material and Methods BEC were collected periodically from 17 volunteers of undergraduate programs, who had participated in practical anatomy classes, before and after FA exposure. Cells were stained according to Feulgen method and then micronucleus test was applied. A total of 1,500 cells were assessed per individual in this study for the micronucleus frequency and other parameters of cytotoxicity. Results There was statistically significant increase in number of micronucleated BEC after FA exposure (after 1 month p=.034 and after 3.5 months p=.017). However, FA exposure caused no significant increase in other nuclear alterations closely related to cytotoxicity (p≥.05). Conclusions FA induced mutagenicity during anatomy classes. Cell death increased, but it was not statistically significant. Efforts have to be made to improve air quality and reduce exposures during anatomy classes. Key words:Carcinogens, formaldehyde, micronucleus tests, mutagenicity tests. |
| Databáze: | OpenAIRE |
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