Increase of MMP-13 expression in multi-stage oral carcinogenesis and epigallocatechin-3-gallate suppress MMP-13 expression
| Autor: | Chiang Wc, Yong-Kie Wong, Chia-Yuan Liu, Kuo Wei Chang, Shu-Chun Lin |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Blotting Western Matrix metalloproteinase Biology Matrix Metalloproteinase Inhibitors medicine.disease_cause Catechin Matrix Metalloproteinase 13 medicine Extracellular Tumor Cells Cultured Neoplasm Anticarcinogenic Agents Humans Protease Inhibitors Collagenases RNA Messenger General Dentistry Tumor marker Aged Neoplasm Staging Reverse Transcriptase Polymerase Chain Reaction Middle Aged medicine.disease Immunohistochemistry stomatognathic diseases Otorhinolaryngology Cell culture Tumor progression Cancer research Carcinoma Squamous Cell Electrophoresis Polyacrylamide Gel Mouth Neoplasms Carcinogenesis |
| Zdroj: | Oral diseases. 12(1) |
| ISSN: | 1354-523X |
| Popis: | Background: Matrix metalloproteinases (MMPs) play pivotal roles in tumor progression. MMP-13 (collagenase-3) digests collagen and other extracellular components. Materials and Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and zymograph were used to study the roles of MMP-13 during the neoplastic process of oral squamous cell carcinoma (OSCC). Results: Increase of MMP-13 mRNA and protein expression in OSCC cell lines relative to cultivated normal oral keratinocytes was found. MMP-13 mRNA expression in OSCC was significantly higher than in non-cancerous match tissue (NCMT) in 36 tissue pairs. Esophageal squamous cell carcinoma also exhibited high MMP-13 mRNA expression. The percentage of OSCC exhibiting strong MMP-13 immunoreactivity was significantly higher than pre-invasive lesion and NCMT. Treatment with >5 μm epigallocatechin-3-gallate (EGCG) to OEC-M1 cells suppressed the expression and activity of MMP-13. Conclusion: MMP-13 could be a potential tumor marker for OSCC. The effects of EGCG in tumor inhibition may act partially through the modulation of MMP-13. |
| Databáze: | OpenAIRE |
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