3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet
| Autor: | Fernando Goglia, Anna Maria Salzano, Maria Moreno, Assunta Lombardi, Andrea Scaloni, Davide Lattanzi, Antonia Giacco, Rita De Matteis, Michele Ceccarelli, Rosalba Senese, Federica Cioffi, Pieter de Lange, Elena Silvestri, Antonia Lanni |
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| Přispěvatelé: | Silvestri, E, Senese, R, Cioffi, F, De Matteis, R, Lattanzi, D, Lombardi, A, Giacco, A, Salzano, Am, Scaloni, A, Ceccarelli, M, Moreno, M, Goglia, F, Lanni, A, de Lange, P., Silvestri, Elena, Senese, Rosalba, Cioffi, Federica, De Matteis, Rita, Lattanzi, Davide, Lombardi, Assunta, Giacco, Antonia, Salzano, Anna Maria, Scaloni, Andrea, Ceccarelli, Michele, Moreno, Maria, Goglia, Fernando, Lanni, Antonia, de Lange, Pieter |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine hormone sensitive lipase medicine.medical_specialty Proteome Diiodothyronines Adipose tissue lcsh:TX341-641 030209 endocrinology & metabolism Hormone-sensitive lipase White adipose tissue Intra-Abdominal Fat Diet High-Fat Weight Gain medicine.disease_cause Article 3 5-diiodo-L-thyronine visceral white adipose tissue ATGL lipolysis proteomics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Adipocyte medicine Animals Lipolysis Protein Interaction Maps Rats Wistar Beta oxidation Nutrition and Dietetics Proteomic Lipid Metabolism 5-diiodo-L-thyronine Lipolysi Rats 030104 developmental biology Endocrinology chemistry Thyronine lcsh:Nutrition. Foods and food supply Oxidative stress Food Science |
| Zdroj: | Nutrients 11 (2019). doi:10.3390/nu11020278 info:cnr-pdr/source/autori:Silvestri E.; Senese R.; Cioffi F.; De Matteis R.; Lattanzi D.; Lombardi A.; Giacco A.; Salzano A.M.; Scaloni A.; Ceccarelli M.; Moreno M.; Goglia F.; Lanni A.; de Lange P./titolo:3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet/doi:10.3390%2Fnu11020278/rivista:Nutrients/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 Nutrients Volume 11 Issue 2 Nutrients, Vol 11, Iss 2, p 278 (2019) |
| DOI: | 10.3390/nu11020278 |
| Popis: | When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 &mu g/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser563, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser563, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health. |
| Databáze: | OpenAIRE |
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