Alarmingly poor performance in Chlamydia trachomatis point-of-care testing
| Autor: | Laura van Dommelen, Sander Ouburg, Christian J. P. A. Hoebe, Peter Terporten, Elfi E. H. G. Brouwers, Frank H. van Tiel, Paul H. M. Savelkoul, Cathrien A. Bruggeman, Servaas A. Morré |
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| Přispěvatelé: | Medical Microbiology and Infection Prevention, Pathology, CCA - Innovative therapy, RS: CARIM School for Cardiovascular Diseases, RS: CAPHRI School for Public Health and Primary Care, Med Microbiol, Infect Dis & Infect Prev, Promovendi NTM, Maastricht University Medical Centre (MUMC), Maastricht University [Maastricht], VU University Medical Center [Amsterdam], South Limbug Public Health Service, South Limburg Public Health Service |
| Rok vydání: | 2010 |
| Předmět: |
Sexually transmitted disease
Adult medicine.medical_specialty Delayed Diagnosis Adolescent Point-of-care testing Point-of-Care Systems Population Chlamydia trachomatis Dermatology medicine.disease_cause Sensitivity and Specificity Young Adult Internal medicine medicine Humans education Reagent Strips Vaginal Smears education.field_of_study Chlamydia biology business.industry Nucleic acid amplification technique Gold standard (test) Chlamydia Infections Middle Aged medicine.disease biology.organism_classification SCREENING SAME DAY TESTING PCR Infectious Diseases Chlamydiales Immunology Female STD CONTROL business Nucleic Acid Amplification Techniques |
| Zdroj: | Sexually Transmitted Infections, 86(5), 355-359. BMJ Publishing Group van Dommelen, L, van Tiel, F H, Ouburg, S, Brouwers, E E H G, Terporten, P H W, Savelkoul, P H M, Morre, S A, Bruggeman, C A & Hoebe, C J P A 2010, ' Alarmingly poor performance in Chlamydia trachomatis point-of-care testing ', Sexually Transmitted Infections, vol. 86, no. 5, pp. 355-359 . https://doi.org/10.1136/sti.2010.042598 Sexually Transmitted Infections Sexually Transmitted Infections, BMJ Publishing Group, 2010, 86 (5), pp.355. ⟨10.1136/sti.2010.042598⟩ |
| ISSN: | 1368-4973 1472-3263 |
| Popis: | Background Infection by Chlamydia trachomatis (CT) is the most prevalent sexually transmitted infection (STI) world wide. The most frequently used diagnostic test for CT is a nucleic acid amplification test (NAAT), which is highly sensitive and specific. To further shorten time delay until diagnosis has been made, in order to prevent CT spread, the use of point-of-care (POC) tests may be the way forward. Objectives The diagnostic performance of three POC tests, Handilab-C, Biorapid CHLAMYDIA Ag test and QuickVue Chlamydia test, was evaluated and compared with NAAT. Methods All women, above the age of 16 years, attending for a consultation at an STI clinic between September 2007 and April 2008, were asked to participate. Women were asked to complete a short questionnaire and to collect six self-taken vaginal swabs (SVS). SVS 2 was used for NAAT and SVS 3 to 5 were randomised for the different POC tests. SVS 1 and 6 were used for determining quantitative CT load to validate the use of successive SVS. All POC tests were performed without knowledge of NAAT results. NAAT was used as the 'gold standard'. Results 772 women were included. CT prevalence was 11% in our population. Sensitivities of the Biorapid CHLAMYDIA Ag test, QuickVue Chlamydia and Handilab-C test were 17%, 27% and 12%, respectively. Conclusions The evaluated POC tests, owing to their very low sensitivities, are not ready for widespread use. These results underline the need for good-quality assurance of POC tests, especially in view of the increased availability of these tests on the internet. |
| Databáze: | OpenAIRE |
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