HMGA2 overexpression plays a critical role in the progression of esophageal squamous carcinoma

Autor: Ivanir Martins, Luis Felipe Ribeiro Pinto, Nathalia Meireles Da Costa, Antonio Palumbo, Alfredo Fusco, Luiz Eurico Nasciutti, Daniela D'Angelo, Francesco Esposito, Vanessa Paiva Leite de Sousa, Marco De Martino
Přispěvatelé: Palumbo, Antonio, Da Costa, Nathalia Meirele, Esposito, Francesco, DE MARTINO, Marco, D'Angelo, Daniela, De Sousa, Vanessa Paiva Leite, Martins, Ivanir, Nasciutti, Luiz Eurico, Fusco, Alfredo, Pinto, Luis Felipe Ribeiro
Rok vydání: 2016
Předmět:
Zdroj: Oncotarget 7 (2016): 25872–25884. doi:10.18632/oncotarget.8288
info:cnr-pdr/source/autori:Palumbo A.; Da Costa N.M.; Esposito F.; De Martino M.; D'Angelo D.; De Sousa V.P.L.; Martins I.; Nasciutti L.E.; Fusco A.; Pinto L.F.R./titolo:HMGA2 overexpression plays a critical role in the progression of esophageal squamous carcinoma/doi:10.18632%2Foncotarget.8288/rivista:Oncotarget/anno:2016/pagina_da:25872/pagina_a:25884/intervallo_pagine:25872–25884/volume:7
Oncotarget
ISSN: 1949-2553
DOI: 10.18632/oncotarget.8288
Popis: // Antonio Palumbo Jr 1, 2 , Nathalia Meireles Da Costa 1 , Francesco Esposito 3 , Marco De Martino 3 , Daniela D’Angelo 3 , Vanessa Paiva Leite de Sousa 1 , Ivanir Martins 4 , Luiz Eurico Nasciutti 2 , Alfredo Fusco 1, 3 , Luis Felipe Ribeiro Pinto 1 1 Programa de Carcinogenese Molecular, Instituto Nacional de Câncer - INCA, Rio de Janeiro, RJ, Brazil 2 Laboratorio de Interacoes Celulares, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro Predio de Ciencias da Saude - Cidade Universitaria, Ilha do Fundao, Rio de Janeiro, RJ, Brazil 3 Istituto di Endocrinologia e Oncologia Sperimentale - CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universita degli Studi di Napoli “Federico II”, Naples, Italy 4 Divisao de Patologia, Instituto Nacional de Câncer - INCA, Rio de Janeiro, RJ, Brazil Correspondence to: Luis Felipe Ribeiro Pinto, email: lfrpinto@inca.gov.br Keywords: esophageal cancer, HMGA proteins, cancer progression, diagnostic marker, malignant phenotype reversion Received: October 05, 2015 Accepted: March 11, 2016 Published: March 23, 2016 ABSTRACT Esophageal Squamous Cell Carcinoma (ESCC) is the most common esophageal tumor worldwide. However, there is still a lack of deeper knowledge about biological alterations involved in ESCC development. High Mobility Group A (HMGA) protein family has been related with poor outcome and malignant cell transformation in several tumor types. In this way, the aim of this study was to analyze the expression of HMGA1 and HMGA2 expression in ESCC and their role in crucial cellular features. We evaluated HMGA1 and HMGA2 mRNA expression in 52 paired ESCC and normal surrounding tissue samples by qRT-PCR. Here, we show that HMGA2, but not HMGA1, is overexpressed in ESCC samples. This result was further confirmed by the immunohistochemical analysis. Indeed, accordingly to mRNA expression data, HMGA2, but not HMGA1, was overexpressed in approximately 90% of ESCC samples, while it was barely expressed in the respective control. Conversely, HMGA1, but not HMGA2, was overexpressed in esophageal adenocarcinoma samples. Interestingly, HMGA2 abrogation attenuated the malignant phenotype of two ESCC cell lines, suggesting that HMGA2 overexpression is involved in ESCC progression.
Databáze: OpenAIRE
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