Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts
| Autor: | Aurora Bernal, Beatriz de Lucas, Beatriz G. Gálvez, Anke Kadow-Romacker, Nuria San Martín, Gustavo R. Plaza, Kay Raum, Laura M. Pérez |
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| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty RHOA Medicina Cell Survival Heart Ventricles Cellular differentiation Enfermedad cardiovascular Cell- and Tissue-Based Therapy Myocardial Infarction Smad Proteins Stimulation Cardiología Low-intensity pulsed ultrasound Cell therapy Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement medicine Animals Humans Progenitor cell Cells Cultured Sistema cardiovascular Ultrasonography 030304 developmental biology 0303 health sciences Corazón biology Gene Expression Profiling Stem Cells Cell Differentiation Bone Morphogenetic Protein Receptors Cell Biology 3. Good health Cell biology Ultrasonic Waves 030220 oncology & carcinogenesis Azacitidine biology.protein Stem cell Signal Transduction |
| Zdroj: | Stem Cell Reviews and Reports, ISSN 1550-8943, 2015-12, Vol. 11, No. 6 Archivo Digital UPM Universidad Politécnica de Madrid |
| ISSN: | 1558-6804 1550-8943 |
| DOI: | 10.1007/s12015-015-9608-6 |
| Popis: | Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive form of mechanical energy that can be delivered into biological tissues as acoustic pressure waves, and is widely used for clinical applications including bone fracture healing. We hypothesized that the positive effects of LIPUS on bone and soft tissue, such as increased cell differentiation and cytoskeleton reorganization, could be applied to increase the therapeutic potential of mesoangioblasts for heart repair. In this work, we show that LIPUS stimulation of cardiac mesoangioblasts isolated from mouse and human heart results in significant cellular modifications that provide beneficial effects to the cells, including increased malleability and improved motility. Additionally, LIPUS stimulation increased the number of binucleated cells and induced cardiac differentiation to an extent comparable with 5′-azacytidine treatment. Mechanistically, LIPUS stimulation activated the BMP-Smad signalling pathway and increased the expression of myosin light chain-2 together with upregulation of β1 integrin and RhoA, highlighting a potentially important role for cytoskeleton reorganization. Taken together, these results provide functional evidence that LIPUS might be a useful tool to explore in the field of heart cell therapy. SAF 2010 3.111 JCR (2015) Q2, 42/124 Medicine, research & experimental; Q3, 14/21 Cell & tissue engineering, 97/187 Cell biology UEM |
| Databáze: | OpenAIRE |
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