Effect of extracellular ATP and UTP on fluid transport by human nasal epithelial cells in culture
| Autor: | S de Bentzmann, Rachid Benali, Denis Pierrot, E. Puchelle, J.M. Zahm |
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| Rok vydání: | 1994 |
| Předmět: |
Intracellular Fluid
Pulmonary and Respiratory Medicine medicine.medical_specialty Cell Membrane Permeability Clinical Biochemistry Uridine Triphosphate 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid Epithelium Membrane Potentials Amiloride chemistry.chemical_compound Adenosine Triphosphate Nasal Polyps Chlorides Internal medicine Tumor Cells Cultured medicine Extracellular Humans Molecular Biology Transepithelial potential difference Uridine triphosphate Ion Transport Epithelial Cells Cell Biology Fluid transport Nasal Mucosa medicine.anatomical_structure Endocrinology chemistry Biophysics Extracellular Space Uracil nucleotide Adenosine triphosphate medicine.drug |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 10:363-368 |
| ISSN: | 1535-4989 1044-1549 |
| Popis: | We have investigated the effect of both adenosine triphosphate (ATP) and uridine triphosphate (UTP) on the fluid transport, transepithelial electric potential difference (PD), and unidirectional chloride flux when applied apically to cultured human surface respiratory epithelial (HSRE) cells in a double-compartment chamber. The effects of ATP and UTP (both 100 microM) were examined in cells either untreated or pretreated with 100 microM amiloride in lactated Ringer's solution. ATP or UTP was added to the apical solution in a 100 microliters final volume. After a 2-h incubation period, the change in fluid transport was measured by weighing the apical fluid. Compared with control, amiloride blocked the fluid absorption by HSRE cells. The addition of ATP or UTP, either alone or after pretreatment with amiloride, induced a similar and significant increase in the apical fluid and chloride flux (P0.001 and P0.005, respectively). The changes in both fluid transport and chloride flux were accompanied by changes in PD. A blocker for chloride transport, 4,4'-diisothiocyano-2,2'-stilbene disulfonate, at 500 microM significantly blocked the ATP-stimulated fluid transport (P0.05) and chloride flux (P0.01). These results support the hypothesis that extracellular ATP and UTP increase the fluid transport by respiratory epithelial cells and may be useful in the hydration of mucus and respiratory mucosa. |
| Databáze: | OpenAIRE |
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