Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment
| Autor: | Damaris Krust, Georg Müller, Sahar Akaberi, Christian Gusbeth, Wolfgang Frey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Technology Environmental Engineering Protein Extraction efficiency Biomass Bioengineering macromolecular substances Arthrospira platensis biomass 010501 environmental sciences 01 natural sciences Incubation period 010608 biotechnology Electric field Pulsed electric field treatment Suspension (vehicle) Waste Management and Disposal Incubation 0105 earth and related environmental sciences Downstream processing Chromatography Renewable Energy Sustainability and the Environment Chemistry Extraction (chemistry) General Medicine Yield (chemistry) C-Phycocyanin ddc:600 |
| Zdroj: | Bioresource technology, 306, 123099 |
| ISSN: | 0960-8524 |
| DOI: | 10.5445/ir/1000117787/post |
| Popis: | Pulsed electric field (PEF) was conducted for the extraction of proteins/C-Phycocyanins from Arthrospira platensis. The cyanobacterial suspension was treated with 1 µs long pulses at an electric field strength of 40 kV·cm−1 and a treatment energy of 114 kJ·kgsus−1 and 56 kJ·kgsus−1. For benchmarking, additional biomass was processed by high pressure homogenization. Homogeneity of the suspension prior to PEF-treatment influenced the protein/C-phycocyanin extraction efficiency. Stability of C-Phycocyanin during post-PEF incubation time was affected by incubation temperature and pH of the external medium. Biomass concentration severely affect proteins/C-Phycocyanins extraction yield via PEF-treatment. The optimum conditions for extraction of proteins/C-Phycocyanin was obtained at 23 °C while incubating in pH 8-buffer. The energy demand for PEF-treatment amounts to 0.56 MJ·kgdw−1 when processing biomass at 100 gdw·kgsus−1. PEF treatment enhances the protein/C-Phycocyanin extraction yield, thus, it can be suggested as preferential downstream processing method for the production of C-Phycocyanin from A. platensis biomass. |
| Databáze: | OpenAIRE |
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