Compensatory regrowth of the mouse bladder after partial cystectomy
| Autor: | Diana K. Bowen, Paula R. Firmiss, Natalie Kukulka, Jessica Wetterlin, Robert W. Dettman, Megan Y. Devine, Grace Delos Santos, Edward M. Gong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Detrusor muscle Physiology medicine.medical_treatment 030232 urology & nephrology lcsh:Medicine urologic and male genital diseases Biochemistry Mice 0302 clinical medicine Fibrosis Immune Physiology Medicine and Health Sciences Morphogenesis lcsh:Science Musculoskeletal System Staining Innate Immune System Smooth Muscles Multidisciplinary Urinary bladder medicine.diagnostic_test Muscles Cystometry Specimen preparation and treatment female genital diseases and pregnancy complications medicine.anatomical_structure Cytokines Female Collagen Anatomy Muscle Regeneration Research Article medicine.medical_specialty Stromal cell Bladder Immunology Urinary Bladder Urology Surgical and Invasive Medical Procedures Cystectomy Urinary System Procedures 03 medical and health sciences Cicatrix medicine Regeneration Animals Surgical Excision business.industry Regeneration (biology) Mesenchymal stem cell lcsh:R DAPI staining Biology and Life Sciences Proteins Renal System Recovery of Function Molecular Development medicine.disease Research and analysis methods 030104 developmental biology Gene Expression Regulation Immune System Nuclear staining lcsh:Q business Collagens Organism Development Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 11, p e0206436 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Cystectomy is the removal of all or part of the urinary bladder. It has been observed that there is significant regrowth of the bladder after partial cystectomy and this has been proposed to be through regeneration of the organ. Regrowth of tissue in mammals has been proposed to involve compensatory mechanisms that share many characteristics of true regeneration, like the growth of specialized structures such as blood vessels or nerves. However, the overall structure of the normal organ is not achieved. Here we tested if bladder growth after subtotal cystectomy (STC, removal of 50% of the bladder) was compensatory or regenerative. To do this we subjected adult female mouse bladders to STC and assessed regrowth using several established cellular parameters including histological, gene expression, cytokine accumulation and cell proliferation studies. Bladder function was analyzed using cystometry and the voiding stain on paper (VSOP) technique. We found that STC bladders were able to increase their ability to hold urine with the majority of volume restoration occurring within the first two weeks. Regenerating bladders had thinner walls with less mean muscle thickness, and they showed increased collagen deposition at the incision as well as throughout the bladder wall suggesting that fibrosis was occurring. Cell populations differed in their response to injury with urothelial regeneration complete by day 7, but stromal and detrusor muscle still incomplete after 8wks. Cells incorporated EdU when administered at the time of surgery and tracing of EdU positive cells over time indicated that many newborn cells originate at the incision and move mediolaterally. Basal urothelial cells and bladder mesenchymal stem cells but not smooth muscle cells significantly incorporated EdU after STC. Since anti-inflammatory cytokines play a role in regeneration, we analyzed expressed cytokines and found that no anti-inflammatory cytokines were present in the bladder 1wk after STC. Our findings suggest that bladder regrowth after cystectomy is compensatory and functions to increase the volume that the bladder can hold. This finding sets the stage for understanding how the bladder responds to cystectomy and how this can be improved in patients after suffering bladder injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |