Cloning and Characterization of the Murine Activin Receptor-like Kinase-1 (ALK-1) Homolog
| Autor: | Claudius E. Robinson, J.M. Gimble, H.W. Fong, Bruce A. Roe, Judy S. Crabtree, Xiying Wu, B.R. Rodriguez |
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| Rok vydání: | 1995 |
| Předmět: |
Stromal cell
Transcription Genetic Activin Receptors Blotting Western Molecular Sequence Data Biophysics Bone Marrow Cells Protein Serine-Threonine Kinases Biology Kidney Polymerase Chain Reaction Biochemistry Antibodies Cell Line law.invention Mice Bone Marrow law Complementary DNA HSPA2 Animals Humans Amino Acid Sequence Cloning Molecular Lung Molecular Biology DNA Primers Base Sequence Sequence Homology Amino Acid Kinase Goats Alternative splicing Brain Cell Biology Molecular biology Recombinant Proteins Organ Specificity Polyclonal antibodies Protein Biosynthesis Recombinant DNA biology.protein Peptides Spleen ACVR2B |
| Zdroj: | Biochemical and Biophysical Research Communications. 216:78-83 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.2594 |
| Popis: | Multiple serine/threonine kinases were detected in a bone marrow stromal cell line. One of these, the murin a ctivin receptor l ike k inase-1 (ALK-1) homolog was cloned and sequenced. The expressed recombinant protein (62 kDa) was consistent in size with that predicted by the cDNA (58.6 kDa). On Western blots, a goat polyclonal antibody detected the native ALK-1 protein in bone marrow stromal cells, lung, brain, kidney and spleen. Two protein species of 60 kDa and 72-76 kDa were detected. Glycosylation events or alternative splicing may account for the larger protein specie. |
| Databáze: | OpenAIRE |
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