Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses
| Autor: | Zhu-Nan Li, Joy Schwerzmann, Daniel B. Jernigan, Kathy Hancock, Megan McCausland, James Stevens, Andrew J. Phipps, Joseph D. Miller, Jacqueline M. Katz, Kimberly M. Weber, Min Z. Levine, Jens Wrammert, Bobbi Jo Horne, Rebecca A. Limmer |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Adolescent Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Biology medicine.disease_cause Antibodies Viral Sensitivity and Specificity Virus Article 03 medical and health sciences Young Adult Antigen Virology Influenza Human medicine Seroprevalence Humans Multiplex Serologic Tests Child Aged Aged 80 and over Hemagglutination assay Assay sensitivity Middle Aged Influenza A virus subtype H5N1 High-Throughput Screening Assays 030104 developmental biology Child Preschool biology.protein |
| Zdroj: | J Virol Methods |
| ISSN: | 1879-0984 |
| Popis: | Influenza hemagglutination inhibition (HI) and virus microneutralization assays (MN) are widely used for seroprevalence studies. However, these assays have limited field portability and are difficult to fully automate for high throughput laboratory testing. To address these issues, three multiplex influenza subtype-specific antibody detection assays were developed using recombinant hemagglutinin antigens in combination with Chembio, Luminex®, and ForteBio® platforms. Assay sensitivity, specificity, and subtype cross-reactivity were evaluated using a panel of well characterized human sera. Compared to the traditional HI, assay sensitivity ranged from 87% to 92% and assay specificity in sera collected from unexposed persons ranged from 65% to 100% across the platforms. High assay specificity (86–100%) for A(H5N1) rHA was achieved for sera from exposed or unexposed to hetorosubtype influenza HAs. In contrast, assay specificity for A(H1N1)pdm09 rHA using sera collected from A/Vietnam/1204/2004 (H5N1) vaccinees in 2008 was low (22–30%) in all platforms. Although cross-reactivity against rHA subtype proteins was observed in each assay platform, the correct subtype specific responses were identified 78% to 94% of the time when paired samples were available for analysis. These results show that high throughput and portable multiplex assays that incorporate rHA can be used to identify influenza subtype specific infections. |
| Databáze: | OpenAIRE |
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