Sterol isomerase HYDRA1 interacts with RNA silencing suppressor P1b and restricts potyviral infection
Autor: | Juan Antonio García, Bernardo Rodamilans, Carmen Simón-Mateo, Mar Martín-Trillo, Jon Ochoa, Adrian Valli |
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Přispěvatelé: | European Commission, Ministerio de Economía y Competitividad (España) |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Agroinfiltration Physiology Green Fluorescent Proteins Arabidopsis Gene Expression Steroid Isomerases Plant Science 01 natural sciences Virus 03 medical and health sciences Bimolecular fluorescence complementation Ipomovirus Plant defense Two-Hybrid System Techniques Tobacco Plant defense against herbivory P1b Plant Diseases biology Potyviridae Temperature Potyvirus biology.organism_classification Isoprenoids Up-Regulation Cell biology Plant Leaves RNA silencing 030104 developmental biology Plum Pox Virus HYD1 Mutation Capsid Proteins RNA Interference Oxidoreductases Protein Binding 010606 plant biology & botany |
Zdroj: | Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Digital.CSIC. Repositorio Institucional del CSIC instname |
DOI: | 10.13039/501100000780 |
Popis: | Plants use RNA silencing as a strong defensive barrier against virus challenges, and viruses counteract this defence by using RNA silencing suppressors (RSSs). With the objective of identifying host factors helping either the plant or the virus in this interaction, we have performed a yeast two-hybrid screen using P1b, the RSS protein of the ipomovirus Cucumber vein yellowing virus (CVYV, family Potyviridae), as a bait. The C-8 sterol isomerase HYDRA1 (HYD1), an enzyme involved in isoprenoid biosynthesis and cell membrane biology, and required for RNA silencing, was isolated in this screen. The interaction between CVYV P1b and HYD1 was confirmed in planta by Bimolecular Fluorescence Complementation assays. We demonstrated that HYD1 negatively impacts the accumulation of CVYV P1b in an agroinfiltration assay. Moreover, expression of HYD1 inhibited the infection of the potyvirus Plum pox virus, especially when antiviral RNA silencing was boosted by high temperature or by coexpression of homologous sequences. Our results reinforce previous evidence highlighting the relevance of particular composition and structure of cellular membranes for RNA silencing and viral infection. We report a new interaction of an RSS protein from the Potyviridae family with a member of the isoprenoid biosynthetic pathway. FEDER Ministerio de Ciencia e Innovación. Grant Numbers: BIO2013-49053-R, BIO2015-73900-JIN, BIO2016-80572-R |
Databáze: | OpenAIRE |
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