Plasmid-mediated VEGF gene transfer induces cardiomyogenesis and reduces myocardial infarct size in sheep
| Autor: | M Criscuolo, G Vera Janavel, Carlos Melo, Nahuel Fernández, Luis Cuniberti, Rubén P. Laguens, Alberto J. Crottogini, M Papouchado, P Cabeza Meckert, Aníbal A. Mele, Andrés Bercovich |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Mitotic index CIENCIAS MÉDICAS Y DE LA SALUD Angiogenesis Genetic enhancement Myocardial Infarction Infarction Mitosis Neovascularization Physiologic Medicina Clínica Transfection Injections Andrology chemistry.chemical_compound Genetics medicine Animals Regeneration Myocytes Cardiac Molecular Biology Tomography Emission-Computed Single-Photon Sheep Sistemas Cardíaco y Cardiovascular business.industry Myocardium Genetic transfer Genetic Therapy medicine.disease Fibrosis Surgery Vascular endothelial growth factor Vascular endothelial growth factor A chemistry Models Animal Molecular Medicine Arteriogenesis business Plasmids |
| Zdroj: | Gene therapy. 13(15) |
| ISSN: | 0969-7128 |
| Popis: | We have recently reported that in pigs with chronic myocardial ischemia heart transfection with a plasmid encoding the 165 isoform of human vascular endothelial growth factor (pVEGF165) induces an increase in the mitotic index of adult cardiomyocytes and cardiomyocyte hyperplasia. On these bases we hypothesized that VEGF gene transfer could also modify the evolution of experimental myocardial infarct. In adult sheep pVEGF165 (3.8 mg, n=7) or empty plasmid (n=7) was injected intramyocardially 1 h after coronary artery ligation. After 15 days infarct area was 11.3±1.3% of the left ventricle in the VEGF group and 18.2±2.1% in the empty plasmid group (P |
| Databáze: | OpenAIRE |
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