Germline sexual fate is determined by the antagonistic action of dmrt1 and foxl3/foxl2 in tilapia
| Autor: | Xin Zhou, Xueyan Wei, Yibing Li, Minghui Li, Xingyong Liu, Deshou Wang, Shuangshuang Qi, Hesheng Xiao, Baoyue Lu, Shengfei Dai |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
Forkhead Box Protein L2 Male Sex Differentiation Somatic cell medicine.drug_class Mutant Biology medicine.disease_cause Germline 03 medical and health sciences 0302 clinical medicine medicine Animals Molecular Biology 030304 developmental biology 0303 health sciences Mutation Cichlids Sex Determination Processes Sex reversal Phenotype Cell biology medicine.anatomical_structure Estrogen Female 030217 neurology & neurosurgery Germ cell Transcription Factors Developmental Biology |
| Zdroj: | Development. 148 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Germline sexual fate has long been believed to be determined by the somatic environment, but this idea is challenged by recent studies of foxl3 mutants in medaka. Here, we demonstrate that the sexual fate of tilapia germline is determined by the antagonistic interaction of dmrt1 and foxl3, which are transcriptionally repressed in male and female germ cells, respectively. Loss of dmrt1 rescued the germ cell sex reversal in foxl3Δ7/Δ7 XX fish, and loss of foxl3 partially rescued germ cell sex reversal but not somatic cell fate in dmrt1Δ5/Δ5 XY fish. Interestingly, germ cells lost sexual plasticity in dmrt1Δ5/Δ5 XY and foxl3Δ7/Δ7 XX single mutants, as aromatase inhibitor (AI) and estrogen treatment failed to rescue the respective phenotypes. However, recovery of germ cell sexual plasticity was observed in dmrt1/foxl3 double mutants. Importantly, mutation of somatic cell-specific foxl2 resulted in testicular development in foxl3Δ7/Δ7 or dmrt1Δ5/Δ5 mutants. Our findings demonstrate that sexual plasticity of germ cells relies on the presence of both dmrt1 and foxl3. The existence of dmrt1 and foxl3 allows environmental factors to influence the sex fate decision in vertebrates. |
| Databáze: | OpenAIRE |
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