Conjugation of a peptide to mannan and its confirmation by tricine sodium dodecyl sulfate–polyacrylamide gel electrophoresis
| Autor: | Alexios Vlamis-Gardikas, Theodore Tselios, Konstantina Kyrtata, Vasso Apostolopoulos, Maria-Eleni Androutsou, Anthi Tapeinou, John Matsoukas |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Tricine Glycine Biophysics Peptide Cell Biology Biology Biochemistry Peptide Fragments Epitope Staining Myelin oligodendrocyte glycoprotein Mannans carbohydrates (lipids) chemistry.chemical_compound chemistry biology.protein Electrophoresis Polyacrylamide Gel Myelin-Oligodendrocyte Glycoprotein Sodium dodecyl sulfate Molecular Biology Polyacrylamide gel electrophoresis Mannan |
| Zdroj: | Analytical Biochemistry. 485:43-45 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2015.06.010 |
| Popis: | The conjugation of polysaccharides to peptides is essential for antigen delivery and vaccine development. Herein, we show that tricine SDS-PAGE in combination with Coomassie Blue staining was adequate to determine the conjugation efficacy of a peptide (epitope 35-55 of myelin oligodendrocyte glycoprotein) to mannan. In addition, tricine SDS-PAGE and periodic acid-Schiff stains were able to monitor the redox state of mannan. Using the described protocol, more than 99.9% of a peptide containing five lysines at its N-terminus was confirmed conjugated to mannan. |
| Databáze: | OpenAIRE |
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