In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica
| Autor: | Carsten Gründemann, Roman Huber, Carmen Steinborn, Rainer Trittler, Sven Stadlbauer, Olivier Potterat, Ulrich Meyer |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class Equisetum Anti-Inflammatory Agents Pharmaceutical Science Equisetaceae Decoction Anti-inflammatory Analytical Chemistry Flow cytometry 03 medical and health sciences Downregulation and upregulation Drug Discovery medicine Humans Lymphocytes Chromatography High Pressure Liquid Pharmacology medicine.diagnostic_test biology Cytokine Measurement Chemistry Organic Chemistry Silicon Dioxide biology.organism_classification In vitro 030104 developmental biology Complementary and alternative medicine Biochemistry Equisetum arvense Molecular Medicine Quercetin Plant Preparations |
| Zdroj: | Planta Medica. 84:519-526 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0043-123075 |
| Popis: | Equisetum arvense, known as common horsetail, is used for the treatment of inflammatory diseases and is the plant with the highest concentration of silica. Yet it is unknown if the medicinal properties are mediated by its silica content. In the current study, optimal conditions for silica-rich horsetail preparations were identified. Bioactivity of the preparations was analyzed in vitro using flow cytometry-based activity and functionality profiling of primary human lymphocytes as well as cytokine measurement using a classical ELISA technique. Experiments revealed that horsetail preparations suppress activation and proliferation of lymphocytes by an interleukin-2-dependent mechanism. The effect increased with the silica concentration in the decoctions. Lymphocytesʼ polyfunctionality was also influenced, shown by a downregulation of IFN-γ. Analytical profiling by HPLC-UV-MS and bioactivity testing revealed relevant immunosuppressive concentrations of a component that has been identified as isoquercitrin. Our results show that both silica and isoquercitrin are active compounds of horsetail preparations. |
| Databáze: | OpenAIRE |
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