Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia
| Autor: | Antonia Infante, Felipe Pretelt, Derly López, Paula Carolina Guzmán, Darío Londoño, Juan Carlos Pérez, Martha Vizcaíno, Alba Campos, Sandra Quijano, Eliana Arias, Julio Solano, Iliana De los Reyes, Laura Trujillo, Alejandra Cañas, Susana Fiorentino, Lina Constanza Beltran Beltran, Graciela Vélez, María Victoria Herrera, Claudia Cardozo |
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| Rok vydání: | 2017 |
| Předmět: |
Pathology
medicine.medical_specialty Multidisciplinary medicine.diagnostic_test business.industry Myelodysplastic syndromes Pericardial fluid body fluids medicine.disease immunophenotype Lymphoma Leukemia medicine.anatomical_structure Immunophenotyping Bronchoalveolar lavage Chronic leukemia medicine flow cytometry Bone marrow lcsh:Science (General) business lcsh:Q1-390 |
| Zdroj: | Universitas Scientiarum, Vol 22, Iss 2, Pp 123-143 (2017) |
| ISSN: | 2027-1352 0122-7483 |
| DOI: | 10.11144/javeriana.sc22-2.sobf |
| Popis: | Flow cytometry (FCM) was implemented in 2008 at the Pontificia Universidad Javeriana and later at the Hospital Universitario San Ignacio to examine special samples of patients with hematological malignancies and solid tumors other than bone marrow and peripheral blood for diagnosis and monitoring. This study describes the main findings of special sample evaluation over a six-year period. In all, 1070 samples of body fluids from patients with benign and malignant diseases were examined by FCM. These samples were stabilized with TransFixTM and stained with six-color immunophenotyping panels. Samples included cerebrospinal fluid, bronchoalveolar lavage, pleural fluid, pericardial fluid and ascite fluid from patients with acute and chronic leukemia, myelodysplastic syndromes, lymphomas, myeloma, autoimmune diseases, immunodeficiencies and solid tumors, among others. Flow cytometry provided important information for the classification and detection of minimal numbers of tumor cells in leukemia and lymphoma cases. This work represents the first national report describing FCM implementation in special samples for diagnosis and clinical monitoring of patients with malignant and benign pathologies. |
| Databáze: | OpenAIRE |
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