The HPV-16 E5 protein inhibits TRAIL- and FasL-mediated apoptosis in human keratinocyte raft cultures
| Autor: | Johannes Schenkel, Nina Mossadegh, Annette Kohl, Pascal Tomakidi, Kirsten Kabsch, Gerda Komposch, Angel Alonso |
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| Rok vydání: | 2003 |
| Předmět: |
Keratinocytes
Fas Ligand Protein Apoptosis DNA Fragmentation Biology Fas ligand TNF-Related Apoptosis-Inducing Ligand Virology Humans Vector (molecular biology) skin and connective tissue diseases Cells Cultured Membrane Glycoproteins integumentary system Caspase 3 Tumor Necrosis Factor-alpha Transfection Raft Oncogene Proteins Viral Molecular biology HaCaT Infectious Diseases Cell culture Polyclonal antibodies Caspases biology.protein Apoptosis Regulatory Proteins |
| Zdroj: | Intervirology. 47(1) |
| ISSN: | 0300-5526 |
| Popis: | By using raft cultures of the polyclonal HaCaT cell lines stably transfected either with E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as reference, we investigated the effect of the human papillomavirus type 16 (HPV-16) E5 protein on apoptosis. In comparison to conventional monolayer cultures this model system allows analysis of apoptosis under more tissue-like conditions by mimicking the stratified organization of a normal surface epithelium. Apoptosis was triggered either by FasL or TRAIL. Execution of the death program was checked at early and late stages by monitoring procaspase-3 cleavage and DNA fragmentation, respectively. Rafts of E5-expressing keratinocytes were completely protected from apoptosis and showed a background of apoptotic cells as low as the untreated cultures. In contrast, the HaCaT/pMSG cultures revealed a dramatic increase in apoptotic cells upon ligand treatment throughout the epithelial compartment. We conclude that the presence of the HPV-16 E5 protein in our tissue-like model prevents FasL- or TRAIL-mediated apoptosis. |
| Databáze: | OpenAIRE |
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