The Changes of Angiogenesis and Immune Cell Infiltration in the Intra- and Peri-Tumoral Melanoma Microenvironment
| Autor: | Magdalena Uvírová, Dusan Ziak, Vladimir Zidlik, Jana Dvorackova, Svetlana Brychtova |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology Skin Neoplasms Angiogenesis T-Lymphocytes Regulatory Neovascularization lcsh:Chemistry angiogenesis Melanoma lcsh:QH301-705.5 Spectroscopy biology Neovascularization Pathologic FOXP3 Forkhead Transcription Factors hemic and immune systems General Medicine Middle Aged Computer Science Applications Female medicine.symptom Adult medicine.medical_specialty CD3 malignant melanoma chemical and pharmacologic phenomena Catalysis Article Inorganic Chemistry Lymphocytes Tumor-Infiltrating medicine nestin Humans Physical and Theoretical Chemistry Molecular Biology Aged Tumor microenvironment Organic Chemistry microvascular density medicine.disease CD90/Thy1 lcsh:Biology (General) lcsh:QD1-999 Cancer cell Cutaneous melanoma Microvessels biology.protein Thy-1 Antigens |
| Zdroj: | International Journal of Molecular Sciences, Vol 16, Iss 4, Pp 7876-7889 (2015) International Journal of Molecular Sciences Volume 16 Issue 4 Pages 7876-7889 |
| ISSN: | 1422-0067 |
| Popis: | Malignant melanoma (MM) urgently needs identification of new markers with better predictive value than currently-used clinical and histological parameters. Cancer cells stimulate the formation of a specialized tumor microenvironment, which reciprocally affects uncontrolled proliferation and migration. However, this microenvironment is heterogeneous with different sub-compartments defined by their access to oxygen and nutrients. This study evaluated microvascular density (MVD), CD3+ lymphocytes (TILs) and FOXP3+ T-regulatory lymphocytes (Tregs) on formalin-fixed paraffin-embedded tissue sections using light microscopy. We analyzed 82 malignant melanomas, divided according to the AJCC TNM classification into four groups--pT1 (35), pT2 (17), pT3 (18) and pT4 (12)--and 25 benign pigmented nevi. All parameters were measured in both the central areas of tumors (C) and at their periphery (P). A marked increase in all parameters was found in melanomas compared to nevi (p = 0.0001). There was a positive correlation between MVD, TILs, FOXP3+ Tregs and the vertical growth phase. The results show that MVD, TILs and FOXP3+ Tregs substantially influence cutaneous melanoma microenvironment. We found significant topographic differences of the parameters between central areas of tumors and their boundaries. |
| Databáze: | OpenAIRE |
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