Glucocorticoid Control of Rat Growth Hormone Gene Expression: Effect on Cytoplasmic Messenger Ribonucleic Acid Production and Degradation
| Autor: | David G. Gardner, Barry J. Gertz, John D. Baxter |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Cytoplasm
endocrine system medicine.medical_specialty medicine.medical_treatment Immunoblotting Peptide hormone Pituitary neoplasm Biology Tritium Dexamethasone chemistry.chemical_compound Endocrinology Internal medicine Gene expression Tumor Cells Cultured medicine Animals RNA Messenger Molecular Biology Messenger RNA General Medicine Molecular biology Introns Uridine Culture Media Rats Kinetics Steroid hormone Somatropin Gene Expression Regulation chemistry Growth Hormone Triiodothyronine hormones hormone substitutes and hormone antagonists Glucocorticoid Half-Life medicine.drug |
| Zdroj: | Molecular Endocrinology. 1:933-941 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | The effect of the glucocorticoid dexamethasone on the production and degradation of rat GH (rGH) cytoplasmic mRNA was studied in cultured rat pituitary tumor (GC) cells. The incorporation of [3H]uridine into both rGH cytoplasmic mRNA and the pyrimidine nucleotide precursor pool was determined in hormone-treated and control cells. From these measurements glucocorticoid effects on absolute production rates of rGH cytoplasmic mRNA were determined and compared to effects on rGH mRNA accumulation. Rat GH mRNA half-life was then calculated based on a first-order decay model. Rat GH mRNA half-life was also directly assayed by: 1) pulse-chase studies and 2) measuring the kinetics of decay of rGH mRNA in cells after transfer from serum-containing to hormone-deficient media. From these independent analyses rGH mRNA half-life estimates ranged from 28-55 h in different experiments. Within individual experiments there was little variability of rGH mRNA decay rates; glucocorticoids were found not to alter the stability of rGH cytoplasmic mRNA. Glucocorticoid induction of rGH cytoplasmic mRNA accumulation was accounted for solely on the basis of increased mRNA production. |
| Databáze: | OpenAIRE |
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