| Popis: |
Background Fracture healing is regulated by endocrine hormones and biochemical and biophysical factors, including hyaluronic acid (HA), which is a component of the extracellular matrix. The prerequisites for biological responses that promote fracture healing, such as biomechanical conditions, and molecular factors, can be investigated in bone cell cultures. Methods In cell experiments, CCK-8 was used to detect the effect of different concentrations of HA on MC3T3-E1 cells after cell intervention. After 10% of HA interfered with MC3T3-E1 cells, they were cultured in induction medium and observed for their mineralization Impact. Total protein was extracted from cells to observe Runx2 and OCN protein expression. After successful modeling in animal experiments, X-rays of the fracture site were taken to determine bone density at the fracture end. After the fracture site was taken, histological examination was performed and the callus was analyzed quantitatively and qualitatively. Results In this study, HA promoted the formation of calcium nodules and the expression of runt-related transcription factor (Runx)2 and osteocalcin (OCN) proteins in MC3T3-E1 cells; In a rabbit tibial fracture model, HA promoted formation of larger calluses than those in control or sham-treated animals at 2, 4, 6, and 8 weeks ( P < 0.05). Osteophytes were larger in HA-treated than in control animals at 4 and 6 weeks ( P < 0.05). Bone mineral density was greater in HA-treated than in control animals at 4, 6, and 8 weeks ( P < 0.05). Conclusions The results showed that HA promoted the formation of calcified nodules in bone cell cultures and healing of rabbit tibial fractures. |
