Resveratrol and insulin association reduced alveolar bone loss and produced an antioxidant effect in diabetic rats
| Autor: | Howard C. Tenenbaum, Fernanda Vieira Ribeiro, Andréia Manetta Molez, Márcio Zaffalon Casati, Fabiano Ribeiro Cirano, Mônica Grazieli Corrêa, Suzana Peres Pimentel |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.medical_treatment Alveolar Bone Loss medicine.disease_cause Antioxidants Diabetes Mellitus Experimental Bone remodeling Superoxide dismutase 03 medical and health sciences 0302 clinical medicine Osteoprotegerin Internal medicine Diabetes mellitus Animals Insulin Medicine Rats Wistar NADPH oxidase biology business.industry 030206 dentistry medicine.disease Streptozotocin Rats 030104 developmental biology Endocrinology Resveratrol biology.protein Periodontics business Oxidative stress medicine.drug |
| Zdroj: | Journal of Periodontology. 92:748-759 |
| ISSN: | 1943-3670 0022-3492 |
| Popis: | BACKGROUND The present investigation studied the effects of systemic administration of resveratrol (RSV) on the development of experimental periodontitis (EP) and on the release of markers of inflammation, bone metabolism, and oxidative stress in diabetic rats. METHODS Seventy-five male rats were divided into five groups: DM+PLAC: Diabetes Mellitus + placebo solution; DM+INS: DM + insulin therapy; DM+RSV: DM + RSV; DM+RSV+INS: DM + RSV and insulin; NDM: non-diabetic. Streptozotocin was used to induce DM and EP was induced by the placement of a ligature at the fist mandibular and the second maxillary molars. Euthanasia occurred 30 days after the initiation of the study and mandible specimens were subjected for morphometric analysis of bone level. Gingival tissues from mandibular molars were collected for quantification of inflammatory and oxidative stress markers by multiplex assay system and ELISA assay, respectively. Maxillary gingival tissues were processed for real-time polymerase chain reaction (real-time PCR) assessment of markers of bone metabolism and oxidative stress. RESULTS Morphometric analysis revealed greater bone loss in DM+PLAC and DM+INS in comparison to the other treatments (P |
| Databáze: | OpenAIRE |
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