Inhibition of Dengue Virus 2 Replication by Artificial MicroRNAs Targeting the Conserved Regions
| Autor: | Yu Xie, Xue-jun Wang, Li-na He, Hai Huang, Peiwen Xie, Sheng-qi Wang, Xiu-juan Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
viruses
Genetic Vectors Molecular Sequence Data Genome Viral Dengue virus Virus Replication medicine.disease_cause Biochemistry Virus Cell Line Conserved sequence Mice RNA interference Cricetinae Drug Discovery Genetics medicine Animals Humans Vector (molecular biology) Molecular Biology Conserved Sequence Base Sequence biology Lentivirus virus diseases Gene targeting Original Articles Dengue Virus biochemical phenomena metabolism and nutrition biology.organism_classification Virology MicroRNAs Flavivirus Viral replication Gene Targeting Molecular Medicine RNA Interference |
| Zdroj: | Nucleic Acid Therapeutics. 23:244-252 |
| ISSN: | 2159-3345 2159-3337 |
| Popis: | Dengue virus (DENV), a mosquito-borne flavivirus, causes serious diseases and threatens public health in tropical and subtropical areas worldwide. RNA interference (RNAi) is a prevailing strategy for antiviral therapy. In this paper, 6 single artificial microRNAs (amiRNAs) targeting the highly conserved regions of the DENV-2 genome were identified and inhibited virus replication efficiently. Then, effective tandem amiRNAs targeting 2 different DENV-2 genome regions were constructed and expressed simultaneously from a single microRNA-like polycistron to avoid virus variation or mutation escape. Finally, the most high-performance tandem amiRNA was embedded in a lenti-viral vector and inhibited DENV-2 virus replication stably and dose-dependently. Overall, these results indicated that RNAi based on multiple amiRNAs targeting viral conserved regions was an effective approach for improvements of nucleic acid inhibitors of DENV and provided a new therapeutic strategy for DENV infection in humans. |
| Databáze: | OpenAIRE |
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