A Novel Mode of Asymmetric Division Identifies the Fly Neuroglioblast 6-4T
| Autor: | Angela Giangrande, Roberto Bernardoni, Gianluca Ragone |
|---|---|
| Přispěvatelé: | Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Rok vydání: | 2001 |
| Předmět: |
Nervous system
Cell glide/gcm Biology 03 medical and health sciences 0302 clinical medicine Glioblast Neuroblast stem cells medicine Animals [SDV.BDD]Life Sciences [q-bio]/Development Biology Molecular Biology In Situ Hybridization ComputingMilieux_MISCELLANEOUS 030304 developmental biology Gliogenesis 0303 health sciences neural precursors Diptera Neuropeptides nervous system RNA Anatomy differentiation Cell Biology Division (mathematics) Immunohistochemistry asymmetric division Cell biology fly medicine.anatomical_structure gliogenesis Trans-Activators Stem cell neuroglioblast neuroblast 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology Developmental Biology, 2001, 235 (1), pp.74-85. ⟨10.1006/dbio.2001.0296⟩ |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.2001.0296 |
| Popis: | Asymmetric cell divisions and segregation of fate determinants are crucial events in the generation of cell diversity. Fly neuroblasts, the precursors that self-reproduce and generate neurons, represent a clear example of asymmetrically dividing cells. Less is known about how neurons and glial cells are generated by multipotent precursors. Flies provide the ideal model system to study this process. Indeed, neuroglioblasts (NGBs) can be specifically identified and have been shown to require the glide/gcm fate determinant to produce glial cells, which otherwise would become neurons. Here, we follow the division of a specific NGB (NGB6-4T), which produces a neuroblast (NB) and a glioblast (GB). We show that, to generate the glioblast, glide/gcm RNA becomes progressively unequally distributed during NGB division and preferentially segregates. Subsequently, a GB-specific factor is required to maintain glide/gcm expression. Both processes are necessary for gliogenesis, showing that the glial vs. neuronal fate choice is a two-step process. This feature, together with glide/gcm subcellular RNA distribution and the behavior of the NGB mitotic apparatus identify a novel type of division generating cell diversity. |
| Databáze: | OpenAIRE |
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