Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ
| Autor: | Ivan Rodriguez, Tobias Ackels, Benoît von der Weid, Marc Spehr |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Yellow fluorescent protein
Genetically modified mouse Vomeronasal organ vomeronasal receptor Neuroscience (miscellaneous) VNO Olfaction lcsh:RC321-571 lcsh:QM1-695 Cellular and Molecular Neuroscience Vomeronasal receptor medicine ddc:610 Original Research Article lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry formyl peptide receptor Formyl peptide receptor biology lcsh:Human anatomy 3. Good health sensory neurons medicine.anatomical_structure Genetically Engineered Mouse biology.protein vomeronasal organ Neuron Anatomy Neuroscience olfaction |
| Zdroj: | Frontiers in Neuroanatomy Frontiers in neuroanatomy 8, 134 (2014). doi:10.3389/fnana.2014.00134 Frontiers in neuroanatomy Frontiers in Neuroanatomy, Vol 8 (2014) |
| ISSN: | 1662-5129 |
| Popis: | The mouse vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Based on largely monogenic expression of either type 1 or 2 vomeronasal receptors (V1Rs / V2Rs) or members of the formyl peptide receptor (FPR) family, the vomeronasal sensory epithelium harbors at least three neuronal subpopulations. While various neurophysiological properties of both V1R- and V2R-expressing neurons have been described using genetically engineered mouse models, the basic biophysical characteristics of the more recently identified FPR-expressing vomeronasal neurons have not been studied. Here, we employ a transgenic mouse strain that coexpresses an enhanced variant of yellow fluorescent protein together with FPR-rs3 allowing to identify and analyze FPR-rs3-expressing neurons in acute VNO tissue slices. Single neuron electrophysiological recordings allow comparative characterization of the biophysical properties inherent to a prototypical member of the FPR-expressing subpopulation of VNO neurons. In this study, we provide an in-depth analysis of both passive and active membrane properties, including detailed characterization of several types of voltage-activated conductances and action potential discharge patterns, in fluorescently labeled versus unmarked vomeronasal neurons. Our results reveal striking similarities in the basic (electro)physiological architecture of both transgene-expressing and non-expressing neurons, confirming the suitability of this genetically engineered mouse model for future studies addressing more specialized issues in vomeronasal FPR neurobiology. |
| Databáze: | OpenAIRE |
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