Osmotic Regulation of ATA2 mRNA Expression and Amino Acid Transport System A Activity
| Autor: | Angelo F. Borghetti, Mara Bonelli, Alessandro E. Caccamo, Kenneth P. Wheeler, P. G. Petronini, Andrea Cavazzoni, Roberta Alfieri |
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| Rok vydání: | 2001 |
| Předmět: |
Gene isoform
Amino Acid Transport System A Swine Hypertonic Solutions Biophysics Cycloheximide Biology Transfection Biochemistry chemistry.chemical_compound Downregulation and upregulation Protein biosynthesis Animals RNA Messenger Amino acid transporter Amino Acids Molecular Biology Cells Cultured Nucleic Acid Synthesis Inhibitors Protein Synthesis Inhibitors chemistry.chemical_classification Messenger RNA Osmotic concentration Membrane Proteins Biological Transport Cell Biology Water-Electrolyte Balance Molecular biology Amino acid Cell biology chemistry Dactinomycin beta-Alanine Endothelium Vascular Carrier Proteins |
| Zdroj: | Biochemical and Biophysical Research Communications. 283:174-178 |
| ISSN: | 0006-291X |
| Popis: | When porcine endothelial cells were exposed to hypertonicity, both the level of ATA2 (amino acid transporter 2) mRNA and activity of amino acid transport System A increased transiently, peaking after about 6 and 9 h, respectively. Cycloheximide, like actinomycin D, prevented both responses, showing that an earlier step also involves protein synthesis. Withdrawal of hypertonicity after 6 h increased the rate of down regulation. These findings confirm that ATA2 is a major isoform of System A and show that changes in the expression of ATA2 mRNA precede both the induction and subsequent down regulation of transport activity. |
| Databáze: | OpenAIRE |
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