In Vivo Assessment of Hypoxia Levels in Pancreatic Tumors Using a Dual-Modality Ultrasound/Photoacoustic Imaging System
| Autor: | Chia-Hua Tsai, Li-Tzong Chen, Nai-Jung Chiang, Wun-Shaing Wayne Chang, Lun-De Liao, Chiung-Cheng Chuang, De-Fu Jhang, Yuhling Wang, Chia-Hui Tsao |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Article
03 medical and health sciences 0302 clinical medicine Pancreatic tumor In vivo TJ1-1570 medicine Mechanical engineering and machinery Electrical and Electronic Engineering 030304 developmental biology 0303 health sciences Tumor microenvironment business.industry hypoxia hemoglobin oxygenation saturation Mechanical Engineering Ultrasound Hypoxia (medical) medicine.disease Functional imaging ultrasound (US) Control and Systems Engineering photoacoustic (PA) Imaging technology Immunohistochemistry pancreatic tumor medicine.symptom business 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Micromachines Volume 12 Issue 6 Micromachines, Vol 12, Iss 668, p 668 (2021) |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi12060668 |
| Popis: | Noninvasive anatomical and functional imaging has become an essential tool to evaluate tissue oxygen saturation dynamics in preclinical or clinical studies of hypoxia. Our dual-wavelength technique for photoacoustic (PA) imaging based on the differential absorbance spectrum of oxyhemoglobin (oxy-Hb) and deoxyhemoglobin (deoxy-Hb) can quantify tissue oxygen saturation using the intrinsic contrast property. PA imaging of tissue oxygen saturation can be used to monitor tumor-related hypoxia, which is a particularly relevant functional parameter of the tumor microenvironment that has a strong influence on tumor aggressiveness. The simultaneous acquisition of anatomical and functional information using dual-modality ultrasound (US) and PA imaging technology enhances the preclinical applicability of the method. Here, the developed dual-modality US/PA system was used to measure relative tissue oxygenation using the dual-wavelength technique. Tissue oxygen saturation was quantified in a pancreatic tumor mouse model. The differences in tissue oxygenation were detected by comparing pancreatic samples from normal and tumor-bearing mice at various time points after implantation. The use of an in vivo pancreatic tumor model revealed changes in hypoxia at various stages of tumor growth. The US/PA imaging data positively correlated with the results of immunohistochemical staining for hypoxia. Thus, our dual-modality US/PA imaging system can be used to reliably assess and monitor hypoxia in pancreatic tumor mouse models. These findings enable the use of a combination of US and PA imaging to acquire anatomical and functional information on tumor growth and to evaluate treatment responses in longitudinal preclinical studies. |
| Databáze: | OpenAIRE |
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