Efficient adeno-associated virus-mediated gene expression in human placenta-derived mesenchymal cells
| Autor: | Xiaohong Zhang, Tsuneo A. Takahashi, Naohide Yamashita, Takashi Nakaoka, Kenichi Shinomiya, Nobukazu Watanabe, Koichi Igura, Toshihide Nishishita |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Gene Expression Regulation
Viral viruses Genetic enhancement Placenta Immunology Population Green Fluorescent Proteins Myocytes Smooth Muscle Biology Recombinant virus medicine.disease_cause Microbiology Umbilical vein Mesoderm Transduction (genetics) Pregnancy Transduction Genetic Virology Gene expression medicine Humans education Adeno-associated virus education.field_of_study Mesenchymal stem cell Nucleic Acid Hybridization Genetic Therapy Dependovirus Flow Cytometry Molecular biology Recombinant Proteins Luminescent Proteins DNA Viral Female HeLa Cells |
| Zdroj: | Microbiology and immunology. 47(1) |
| ISSN: | 0385-5600 |
| Popis: | Mesenchymal cells from various sources are pluripotent and are attractive sources for cell transplantation. In this study, we analyzed recombinant adeno-associated virus (rAAV)-mediated gene expression in human placenta-derived mesenchymal cells (hPDMCs), which reside in placental villi. After transduction of AV-CAG-EGFP, a rAAV expressing enhanced green fluorescence protein (EGFP), hPDMCs showed much higher level of EGFP expression than human umbilical vein endothelial cells or rat aortic smooth muscle cells. The number of EGFP-positive hPDMCs infected by AV-CAG-EGFP alone did not increase significantly by coinfection of adenovirus, which enhanced expression level of the rAAV vector. Moreover, flow cytometric analysis showed discrete positive fraction of EGFP-expressing hPDMCs, which is about 15-20% of the cells infected with AV-CAG-EGFP. Therefore, some cell population in hPDMCs might be highly susceptible to rAAV-mediated gene transduction. In addition, stable EGFP expressions were observed in about 1% of hPDMCs infected with AV-CAG-EGFP at 4 weeks post-infection. Collectively, hPDMCs have characters favorable for rAAV-mediated gene expression. |
| Databáze: | OpenAIRE |
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