Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells
| Autor: | Aadlen Foudi, Dorothee Buet, Fawzia Louache, Peggy Jarrier, William Vainchenker, Abdelale Jalil, Magali Berthebaud, Yanyan Zhang, Jean-Francois Geay |
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| Rok vydání: | 2004 |
| Předmět: |
Receptors
CXCR4 Stromal cell Endosome Green Fluorescent Proteins CD34 Vesicular Transport Proteins Antigens CD34 Platelet Membrane Glycoproteins Biology Endocytosis Ligands CXCR4 Antigens CD Cell Movement Humans rab5 GTP-Binding Proteins Microscopy Confocal Tetraspanin 30 rab4 GTP-Binding Proteins Chemotaxis Cell Membrane Temperature Colocalization Lysosome-Associated Membrane Glycoproteins Membrane Proteins Cell migration Cell Biology Flow Cytometry Hematopoietic Stem Cells Molecular biology Clathrin Cell biology Retroviridae Gene Expression Regulation rab GTP-Binding Proteins Molecular Medicine Stem cell Developmental Biology Plasmids Signal Transduction |
| Zdroj: | Stem cells (Dayton, Ohio). 22(6) |
| ISSN: | 1066-5099 |
| Popis: | CXCR4, the stromal cell-derived factor-1 receptor, plays an important role in the migration of hematopoietic progenitor/stem cells. The surface and cytoplasmic expression of CXCR4 on human hematopoietic CD34(+) cells was investigated. We show that its surface expression is low, whereas a large part of CXCR4 protein is sequestered intracellularly. Using confocal microscopy, we demonstrated that CXCR4 is colocalized with EEA-1, Rab5, Rab4, and Rab11, which are localized in early and recycling endosomes. No significant colocalization of CXCR4 with lysosomal markers CD63 and Lamp-1 was detected. Using antibody feeding experiments, we report a role for CXCR4 constitutive endocytosis in subcellular localization in stably transduced UT7-CXCR4-GFP and CD34(+) cells. Agonist-independent endocytosis of CXCR4 occurs through clathrin-coated vesicles. These data implicate a constitutive endocytosis in the regulation of CXCR4 membrane expression and suggest that constitutive endocytosis may be involved in the regulation of trafficking the human hematopoietic progenitor/stem cells to and in the bone marrow microenvironment. |
| Databáze: | OpenAIRE |
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