Multimodal interference-based imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm
| Autor: | Greta M. Bauer, Aya Eid, John F. Marko, Luay M. Almassalha, Hariharan Subramanian, Simona Morochnik, Di Zhang, Wenli Wu, John E. Chandler, Guillermo A. Ameer, Scott Gladstein, Vadim Backman, Andrew D. Stephens, Igal Szleifer, Adam Eshein, Lusik Cherkezyan |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Time Factors animal structures Intravital Microscopy Ultraviolet Rays Cellular differentiation Science General Physics and Astronomy Apoptosis 02 engineering and technology Phosphatidylserines Multimodal Imaging General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Humans Microscopy Interference lcsh:Science Millisecond Multidisciplinary Phantoms Imaging Dynamics (mechanics) Cell Differentiation Mesenchymal Stem Cells General Chemistry 021001 nanoscience & nanotechnology Photobleaching Fluorescence Chromatin Actin Cytoskeleton 030104 developmental biology Temporal resolution Biophysics lcsh:Q 0210 nano-technology Intracellular Nanospheres HeLa Cells |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Understanding the relationship between intracellular motion and macromolecular structure remains a challenge in biology. Macromolecular structures are assembled from numerous molecules, some of which cannot be labeled. Most techniques to study motion require potentially cytotoxic dyes or transfection, which can alter cellular behavior and are susceptible to photobleaching. Here we present a multimodal label-free imaging platform for measuring intracellular structure and macromolecular dynamics in living cells with a sensitivity to macromolecular structure as small as 20 nm and millisecond temporal resolution. We develop and validate a theory for temporal measurements of light interference. In vitro, we study how higher-order chromatin structure and dynamics change during cell differentiation and ultraviolet (UV) light irradiation. Finally, we discover cellular paroxysms, a near-instantaneous burst of macromolecular motion that occurs during UV induced cell death. With nanoscale sensitive, millisecond resolved capabilities, this platform could address critical questions about macromolecular behavior in live cells. Methods to track molecular motion in eukaryotic cells mostly rely on fluorescent labels, transfection or photobleaching. Here the authors use multimodal partial wave spectroscopy to perform label-free live cell measurements of nanoscale structure and macromolecular motion with millisecond temporal resolution. |
| Databáze: | OpenAIRE |
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