VISIBLE CHEMILUMINESCENCE ASSOCIATED WITH THE REACTION BETWEEN METHEMOGLOBIN OR OXYHEMOGLOBIN WITH HYDROGEN PEROXIDE
| Autor: | Carlos Pascual, Tais H. Schmitt, María Dolores del Castillo, Jorge A. Escobar, Eduardo Lissi, Paolo Di Mascio |
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| Rok vydání: | 1994 |
| Předmět: |
Hemeprotein
Free Radicals Radical Inorganic chemistry Ascorbic Acid Photochemistry Biochemistry Antioxidants Methemoglobin law.invention chemistry.chemical_compound law Animals Chromans Physical and Theoretical Chemistry Hydrogen peroxide Chemiluminescence Chemistry Free Radical Scavengers Hydrogen Peroxide General Medicine Ascorbic acid Oxyhemoglobins Luminescent Measurements Cattle Trolox Hemin |
| Zdroj: | Photochemistry and Photobiology. 60:405-411 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/j.1751-1097.1994.tb05124.x |
| Popis: | Visible chemiluminescence is emitted in the irreversible deactivation of hemoglobin or methemoglobin with excess H2O2. The emission takes place in two phases. The most intense one lasts a few seconds and is followed by a second phase of lower intensity that remains for longer periods. This second phase presents chaotic or sustained oscillations. Free radicals are implicated in the luminescent process since the emission can be reduced by free radical scavengers such as 6-hydroxy-2,5,7,8,-tetramethylchroman-2-carboxylic acid (Trolox) or ascorbic acid. These additives lead to a delay in reaching the maximum intensity, which can be related to their consumption, implying substantial recycling of the hemoprotein. Chemiluminescence is also observed in the oxidation of hemin by H2O2, suggesting a role for the heme group in the processes leading to the excited state production. The lower intensity observed in the presence of hemin can be related to the contribution of the globin chains. |
| Databáze: | OpenAIRE |
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