In vitro genotoxicity evaluation of acetamiprid in CaCo-2 cells using the micronucleus, comet and γH2AX foci assays
| Autor: | Tolga Çavaş, Mümün Coşkun, Ozgur Vatan, Nilufer Cinkilic, Dilek Yilmaz |
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| Přispěvatelé: | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Çavaş, Tolga, Cinkılıç, Nilufer, Vatan, Özgür, Yılmaz, Dilek, Coşkun, Mümün, AAH-3508-2021, O-7508-2015, AAH-5296-2021 |
| Rok vydání: | 2012 |
| Předmět: |
Biochemistry & Molecular Biology
Physiology In vitro genotoxicity Cytotoxicity Health Toxicology and Mutagenesis Apoptosis Biology Toxicology Relevance medicine.disease_cause Acetamiprid Induction Dna-damage chemistry.chemical_compound Neonicotinoid insecticides medicine Cytotoxic T cell CaCo-2 Pesticides Genotoxic Genetics Toxicity Hexapoda Neonicotinoid General Medicine Molecular biology Micronucleus chemistry Caco-2 Comet Neonicotinoids Imidacloprid Gamma H2AX Entomology Agronomy and Crop Science Genotoxicity Human-lymphocytes |
| Zdroj: | Pesticide Biochemistry and Physiology. 104:212-217 |
| ISSN: | 0048-3575 |
| DOI: | 10.1016/j.pestbp.2012.08.004 |
| Popis: | Acetamiprid is a member of the neonicotinoid group of insecticides commonly used against wide range of insect pests. In the present study, in vitro cytotoxicity and genotoxicity of technical grade acetamiprid was evaluated on the human intestinal CaCo-2 cells. Cytotoxicity was evaluated using the clonogenic survival and the results indicated that acetamiprid was cytotoxic on CaCo-2 cells. The cells were than treated with acetamiprid concentrations exhibit greater than 75% clonogenic survival for 24 h, to assess genotoxicity using the micronucleus, comet and γH2AX foci formation assays. Our results indicate that, under the experimental conditions used, acetamiprid has cytotoxic and genotoxic potential on human intestinal cells. |
| Databáze: | OpenAIRE |
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