Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum
| Autor: | Mary Treinen-Moslen, Mary F. Kanz, Lata Kaphalia, Bhupendra S. Kaphalia, Santosh Kumar |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Diclofenac Metabolite Clinical Biochemistry Biochemistry Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Pharmacokinetics Spectrophotometry medicine Animals Antipyretic Chromatography High Pressure Liquid Detection limit Chromatography medicine.diagnostic_test Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine Rats stomatognathic diseases chemistry Spectrophotometry Ultraviolet Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Chromatography B. 830:231-237 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2005.10.045 |
| Popis: | A rapid and sensitive high-performance liquid chromatographic method was developed for determination of diclofenac and its major metabolite, 4'-hydroxydiclofenac, in serum from rats treated with diclofenac. The method is simple with a one-step extraction procedure, isocratic HPLC separation, and UV detection at 280 nm. Use of N-phenylanthranilic acid as the internal standard provided good accuracy without interference by endogenous compounds or 5-hydroxydiclofenac, another metabolite of interest. Limits of detection for diclofenac and 4'-hydroxydiclofenac were 0.0225 and 0.0112 microg/ml, respectively. Average extraction efficiencies of diclofenac, 4'-hydroxydiclofenac, and the internal standard were >/=76%. The method was applied to serum collected at 3h after rats were treated with an experimentally useful dosage range of 3, 10 and 50mg/kg diclofenac. Recovery (as a percentage of dose) for the 4'-hydroxy metabolite in serum was found to consistently average from 0.10 to 0.12% following each dosage, whereas recovery of diclofenac in serum declined from 0.45 to 0.37%. Thus, the method is suitable for measurement of a major diclofenac metabolite in experimental studies. |
| Databáze: | OpenAIRE |
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