Sweetness characterization of recombinant human lysozyme
| Autor: | Kana Nakajima, Shigezo Udaka, Yutaka Kashiwagi, Mami Matano, Kenji Maehashi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Taste
Physiology Biochemistry Pichia Pichia pastoris law.invention chemistry.chemical_compound law Humans Molecular Biology chemistry.chemical_classification Innate immune system biology digestive oral and skin physiology food and beverages Sweetness biology.organism_classification Recombinant Proteins Enzyme Activation Enzyme chemistry Recombinant DNA Muramidase Lysozyme Breast feeding |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 188:8-14 |
| ISSN: | 1096-4959 |
| Popis: | Lysozyme, a bacteriolytic enzyme, is widely distributed in nature and is a component of the innate immune system. It is established that chicken egg lysozyme elicits sweetness. However, the sweetness of human milk lysozyme, which is vital for combating microbial infections of the gastrointestinal tract of breast-fed infants, has not been characterized. This study aimed to assess the elicitation of sweetness using recombinant mammalian lysozymes expressed in Pichia pastoris. Recombinant human lysozyme (h-LZ) and other mammalian lysozymes of mouse, dog, cat and bovine milk elicited similar sweetness as determined using a sensory test, whereas bovine stomach lysozyme (bs-LZ) did not. Assays of cell cultures showed that h-LZ activated the human sweet taste receptor hT1R2/hT1R3, whereas bs-LZ did not. Point mutations confirmed that the sweetness of h-LZ was independent of enzyme activity and substrate-binding sites, although acidic amino acid residues of bs-LZ played a significant role in diminishing sweetness. Therefore, we conclude that elicitation of sweetness is a ubiquitous function among all lysozymes including mammalian lysozymes. These findings may provide novel insights into the biological implications of T1R2/T1R3-activation by mammalian lysozyme in the oral cavity and gastrointestinal tract. However, the function of lysozyme within species lacking the functional sweet taste receptor gene, such as cat, is currently unknown. |
| Databáze: | OpenAIRE |
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