The in vivo opsonizing activity of Fab μ and F(c)5 μ fragments of pig immunoglobulin M to Escherichia coli 0 55 in newborn, precolostral piglets
| Autor: | Jiří Zukán, Ivo Miler |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Time Factors
Swine Physical Therapy Sports Therapy and Rehabilitation medicine.disease_cause Immunoglobulin Fab Fragments In vivo Escherichia coli medicine Animals Opsonin biology Immunoglobulin mu-Chains Chemistry Colostrum Rehabilitation Immunoglobulin Fc Fragments General Medicine Opsonin Proteins biology.organism_classification In vitro Animals Newborn Immunoglobulin M Biochemistry biology.protein Immunoglobulin Heavy Chains Oxidation-Reduction Bacteria |
| Zdroj: | Immunochemistry. 12:813-815 |
| ISSN: | 0019-2791 |
| DOI: | 10.1016/0019-2791(75)90145-7 |
| Popis: | In vivo opsonizing activity and in vitro bactericidal effect of IgM antibodies and their fragments to E. Coli 0 55 were studied. Total reduction and carboxymethylation of disulfide bonds of the IgM molecules completely abolished its bactericidal and opsonizing activity, suggesting the importance of these bonds for both activities. The peptic Fab μ fragment possessed both bactericidal and opsonizing activity, though a smaller (4200 daltons lessA) tryptic Fab μ fragment was completely inactive. The peptic F(c)5 μ fragment opsonized bacteria in vivo without any bacterial effect in vitro. The possible participation of complement as well as a possibility of nonspecific binding of the peptic F(c)5 μ to bacteria and macrophages is discussed. |
| Databáze: | OpenAIRE |
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