Zebrafish brain RNA sequencing reveals that cell adhesion molecules are critical in brain aging
| Autor: | Özge Pelin Burhan, Naz Şerifoğlu, Fatma Kahveci, Michelle M. Adams, Bihter Muratoğlu, Ayca Arslan-Ergul, Begun Erbaba |
|---|---|
| Přispěvatelé: | Erbaba, Begün, Burhan, Özge Pelin, Şerifoğlu, Naz, Kahveci, Fatma, Adams, Michelle M. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fetal Proteins Aging Cell Adhesion Molecules Neuronal Gene Expression Biology Connexins 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Antigens CD Activated-Leukocyte Cell Adhesion Molecule Gene expression Animals Humans Progenitor cell Gene Zebrafish ALCAM Cell adhesion molecules Cell adhesion molecule Sequence Analysis RNA General Neuroscience Brain atlas RNA Brain Gene Expression Regulation Developmental RNA sequencing biology.organism_classification Cell biology 030104 developmental biology Brain aging Neurology (clinical) Geriatrics and Gerontology Cell Adhesion Molecules 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Neurobiology of Aging |
| ISSN: | 1558-1497 |
| Popis: | Brain aging is a complex process, which involves multiple pathways including various components from cellular to molecular. This study aimed to investigate the gene expression changes in zebrafish brains through young-adult to adult, and adult to old age. RNA sequencing was performed on isolated neuronal cells from zebrafish brains. The cells were enriched in progenitor cell markers, which are known to diminish throughout the aging process. We found 176 statistically significant, differentially expressed genes among the groups, and identified a group of genes based on gene ontology descriptions, which were classified as cell adhesion molecules. The relevance of these genes was further tested in another set of zebrafish brains, human healthy, and Alzheimer's disease brain samples, as well as in Allen Brain Atlas data. We observed that the expression change of 2 genes, GJC2 and ALCAM, during the aging process was consistent in all experimental sets. Our findings provide a new set of markers for healthy brain aging and suggest new targets for therapeutic approaches to neurodegenerative diseases. (C) 2020 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect