Study of the effect of the bacterial and fungal communities present in real wastewatereffluents on the performance of fungal treatments

Autor: Tero Tuomivirta, Marina Badia-Fabregat, Hannu Fritze, Sara Rodríguez-Mozaz, Daniel Lucas, Taina Pennanen, Damià Barceló, Teresa Vicent, Gloria Caminal
Přispěvatelé: Ministerio de Economía y Competitividad (España), Barceló, Damià, Caminal, Glòria, Barceló, Damià [0000-0002-8873-0491], Caminal, Glòria [0000-0001-9646-6099]
Rok vydání: 2021
Předmět:
Zdroj: Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Recercat. Dipósit de la Recerca de Catalunya
instname
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Digital.CSIC. Repositorio Institucional del CSIC
Popis: The use of the ligninolytic fungi Trametes versicolor for the degradation of micropollutants has been widely studied. However, few studies have addressed the treatment of real wastewater containing pharmaceutically active compounds (PhAC) under non-sterile conditions. The main drawback of performing such treatments is the difficulty for the inoculated fungus to successfully compete with the other microorganisms growing in the bioreactor. In the present study, several fungal treatments were performed under non-sterile conditions in continuous operational mode with two types of real wastewater effluent, namely, a reverse osmosis concentrate (ROC) from a wastewater treatment plant and a veterinary hospital wastewater (VHW). In all cases, the setup consisted of two parallel reactors: one inoculated with T. versicolor and one non-inoculated, which was used as the control. The main objective of this work was to correlate the operational conditions and traditional monitoring parameters, such as laccase activity, with PhAC removal and the composition of the microbial communities developed inside the bioreactors. For that purpose a variety of biochemical and molecular biology analyses were performed: phospholipid fatty acids analysis (PLFA), quantitative PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE) followed by sequencing. The results show that many indigenous fungi (and not only bacteria, which were the focus of the majority of previously published research) can successfully compete with the inoculated fungi (i.e., Trichoderma asperellum overtook T. versicolor in the ROC treatment). We also showed that the wastewater origin and the operational conditions had a stronger impact on the diversity of microbial communities developed in the bioreactors than the inoculation or not with T. versicolor. © 2016 Elsevier B.V.
The authors would like to acknowledge the UAB veterinary hospital staff for their kindness permission and assistance with sampling and Michele Stefani, Dr. Quim Comas and Dr. Hèctor Monclús for providing us with the ROC. This work has been funded by the Spanish Ministry of Economy and Competitiveness and FEDER (projects CTQ2010-21776-C02 and CTM2013-48545-C2) and supported by the Generalitat de Catalunya (Consolidated Research Groups 2014-SGR-291 and 2014-SGR-476) and the Academy of Finland Research (grant 292967). The Department of Chemical Engineering of the Universitat Autònoma de Barcelona (UAB) is member of the Xarxa de Referència en Biotecnologia de la Generalitat de Catalunya. M. Badia-Fabregat acknowledges the predoctoral grant from UAB and the Generalitat de Catalunya grant for research stays (2012 BE100857). D. Lucas acknowledges the predoctoral grant from the Spanish Ministry of Education, Culture and Sports (AP-2010-4926). The authors would also like to acknowledge the dedication of the reviewers who contributed to the improvement of the article with their comments.
Databáze: OpenAIRE
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