Differential Cytokine Regulation of MHC Class II and Thyroglobin mRNAs in Rat Thyroid Cells
| Autor: | P. Graves, Terry F. Davies, D. S. Neufeld |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
endocrine system
Transcription Genetic endocrine system diseases medicine.medical_treatment Genes MHC Class II Thyroid Gland Biology Thyroglobulin Cell Line Interferon-gamma Endocrinology Gene expression medicine Animals Interferon gamma RNA Messenger Molecular Biology MHC class II Alternative splicing Thyroid Histocompatibility Antigens Class II General Medicine Blotting Northern Molecular biology Recombinant Proteins Rats Kinetics medicine.anatomical_structure Cytokine Genes Cell culture biology.protein DNA Probes medicine.drug |
| Zdroj: | Molecular Endocrinology. 3:758-762 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | We have previously demonstrated that recombinant gamma-interferon (gamma IF) inhibits thyroid cell proliferation in vitro. We now demonstrate differential regulation of thyroid cell genes by recombinant gamma IF, as evidenced by data obtained measuring thyroglobulin (Tg) mRNA and MHC class II alpha-chain mRNA transcripts. Using the rat thyroid cell clone 1B-6, derived from FRTL-5 cells, gamma IF markedly increases MHC class II (RT1.D) alpha-chain transcripts in proliferating cells. Simultaneously, Tg mRNA transcript levels are markedly inhibited, even in the presence of TSH and insulin-like growth factor I (as insulin), known stimulators of Tg mRNA. Similar data were obtained for both FRTL-5 and FRTL parent cell lines. Furthermore, using probes to the 5' region of the rat Tg gene we recognized not just a 9.0-kilobase (kb) mRNA, but also an additional 1.1-kb message in all proliferating thyroid cell cultures, suggesting truncation or alternative splicing of the Tg mRNA. Both the 9.0- and 1.1-kb mRNAs were inhibited by gamma IF. These data add further complexity to the cytokine regulation of thyroid cell gene expression and advise caution in making the assumption that thyroid cells may be efficient thyroid antigen-presenting cells in thyroid autoimmune disease. |
| Databáze: | OpenAIRE |
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