The Effects of Operational Conditions in Scaling Up of Xylanase Enzyme Production for Xylitol Production

Autor:	Dewinka Anugeraheni, Abdul Aziz, Tjandra Setiadi, Made Tri Ari Penia Kresnowati, Diah Meilany
Rok vydání:	2020
Předmět:	lcsh:TP155-156 food and beverages Industrial fermentation General Medicine Xylose Xylitol Pulp and paper industry carbohydrates (lipids) chemistry.chemical_compound chemistry Enzymatic hydrolysis Xylanase Hemicellulose Fermentation lcsh:Chemical engineering Aeration
Zdroj:	Reaktor, Vol 20, Iss 1, Pp 32-37 (2020)
ISSN:	2407-5973 0852-0798
DOI:	10.14710/reaktor.20.1.32-37
Popis:	The biological route to produce xylitol from Oil Palm Empty Fruit Bunches (EFBs) comprises of EFBs pretreatment, enzymatic hydrolysis, fermentation, and downstream separation of the produced xylitol. Due to the specificity in the hemicellulose composition of EFBs, a xylanase enzyme that has a high affinity to EFBs is required to hydrolyze the EFBs into xylose. In this research, the influences of aeration, humidity, and mixing in xylanase production were mapped. The xylanase production was performed by Aspergillus fumigatus ITBCCL170 in a solid-state fermentation using a tray fermenter with EFBs as the substrate. The optimal configuration was further scaled up into xylanase production using 1000 g of EFBs as the substrate. The results showed that the highest enzyme activity was 236.3 U/g EFB, obtained from the use of humid air airflow of 0.1 LPM, and mixing was performed once a day. The scaling up resulted in a lower xylanase activity and call for a better design of the fermenter.Keywords: aeration, humidity, mixing, OPEFBs, tray fermenter, xylanase, xylitol
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac01bebdbaf1eee318939ab8684d5e20 https://doi.org/10.14710/reaktor.20.1.32-37 Zobrazit plný text záznamu