Volume-activated trimethylamine oxide efflux in red blood cells of spiny dogfish (Squalus acanthias)
| Autor: | Ainsley Vaz MacLean, Dana-Lynn T. Koomoa, Mark W. Musch, Leon Goldstein |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Ethylene Glycol
Taurine Erythrocytes Physiology Trimethylamine 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid Ammonium Chloride Blood cell Methylamines chemistry.chemical_compound Squalus acanthias Physiology (medical) medicine Animals Syk Kinase Urea Enzyme Inhibitors Cell Size Enzyme Precursors Ion Transport Spiny dogfish Dose-Response Relationship Drug Quinine biology Osmolar Concentration Intracellular Signaling Peptides and Proteins Niflumic Acid Protein-Tyrosine Kinases biology.organism_classification Red blood cell src-Family Kinases medicine.anatomical_structure Hypotonic Solutions chemistry Biochemistry Dogfish Osmoregulation Efflux |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 281:R803-R810 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.2001.281.3.r803 |
| Popis: | The aims of this study were to determine the pathway of swelling-activated trimethylamine oxide (TMAO) efflux and its regulation in spiny dogfish ( Squalus acanthias) red blood cells and compare the characteristics of this efflux pathway with the volume-activated osmolyte (taurine) channel present in erythrocytes of fishes. The characteristics of the TMAO efflux pathway were similar to those of the taurine efflux pathway. The swelling-activated effluxes of both TMAO and taurine were significantly inhibited by known anion transport inhibitors (DIDS and niflumic acid) and by the general channel inhibitor quinine. Volume expansion by hypotonicity, ethylene glycol, and diethyl urea activated both TMAO and taurine effluxes similarly. Volume expansion by hypotonicity, ethylene glycol, and diethyl urea also stimulated the activity of tyrosine kinases p72syk and p56lyn, although the stimulations by the latter two treatments were less than by hypotonicity. The volume activations of both TMAO and taurine effluxes were inhibited by tyrosine kinase inhibitors, suggesting that activation of tyrosine kinases may play a role in activating the osmolyte effluxes. These results indicate that the volume-activated TMAO efflux occurs via the organic osmolyte (taurine) channel and may be regulated by the volume activation of tyrosine kinases. |
| Databáze: | OpenAIRE |
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