Imaging growth dynamics of tumour spheroids using optical coherence tomography
| Autor: | Yogesh Verma, Mrinalini Sharma, R. Nair, Pradeep Kumar Gupta, K. D. Rao |
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| Rok vydání: | 2006 |
| Předmět: |
Materials science
genetic structures Bioengineering Cell Count Applied Microbiology and Biotechnology chemistry.chemical_compound Nuclear magnetic resonance Optical coherence tomography Cell Line Tumor Neoplasms Spheroids Cellular Tumour spheroid Microscopy medicine Humans Propidium iodide medicine.diagnostic_test Dynamics (mechanics) Spheroid General Medicine Fluorescence eye diseases chemistry embryonic structures sense organs Tomography Tomography Optical Coherence Biotechnology |
| Zdroj: | Biotechnology letters. 29(2) |
| ISSN: | 0141-5492 |
| Popis: | Optical coherence tomography (OCT) was used to monitor the dynamics of tumour spheroid formation by the hanging drop method. In contrast to microscopy, the estimates obtained using OCT for the volume of the spheroid, were consistent with the measured changes in cell number as a function of time. The OCT images also revealed heterogeneous structures in the spheroids of approximately 200 microm diameter. These corresponded to the necrotic regions identified by fluorescence of propidium iodide stained cells. |
| Databáze: | OpenAIRE |
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