A point of confusion for embryologists in the identification of viable spermatozoa by the eosin-nigrosin test
| Autor: | Hong Zhou, Caizhu Wang, Xianyou Gan, Jinhui Shu, Chen Huanhua, Ruoyun Lin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
endocrine system
medicine.medical_treatment Laser Case Report 030209 endocrinology & metabolism Semen Semen analysis Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization medicine reproductive and urinary physiology 030219 obstetrics & reproductive medicine Assisted reproductive technology medicine.diagnostic_test Spermatozoon urogenital system business.industry Sperm Nigrosin medicine.anatomical_structure Reproductive Medicine Immotile spermatozoa business Eosin-nigrosin test |
| Zdroj: | Clinical and Experimental Reproductive Medicine |
| ISSN: | 2233-8241 2233-8233 |
| Popis: | A viable spermatozoon is a prerequisite for fertilization in intracytoplasmic sperm injection (ICSI). Thus, it is crucial to select viable but immotile spermatozoa on the day of ICSI. We report conflicting results in the identification of viable but immotile spermatozoa between the eosin-nigrosin staining and the laser test, which resulted in confusion for embryologists during assisted reproductive technology (ART). Three patients’ semen samples that showed no motile spermatozoa are described in this report. To identify viable spermatozoa, we used both the eosin-nigrosin test and the laser test for each sample, and repeated the semen analysis twice in each patient. Viable but immotile spermatozoa selected by the laser test were used for ICSI. Viable spermatozoa were detected by both the eosin-nigrosin and laser tests in two patients (case 1, 95.00% vs. 24.21% and 92.68% vs. 22.22%; case 2, 41.18% vs. 23.48% and 39.81% vs. 22.52%), indicating consistent results between the two methods. In the third patient, the eosin-nigrosin test yielded viability rates of 20.75% and 19.14%, while the result of the laser test was 0%. Thus, testicular aspiration was performed to collect viable sperm from this patient. Normal fertilization was achieved after the injection of viable but immotile spermatozoa selected from these patients by the laser test, resulting in the birth of two healthy babies. Our study documents a case where the eosin-nigrosin test showed a limitation in identifying viable but immotile spermatozoa for ART, while the laser test may overcome this limitation. Larger samples may be required to corroborate the clinical value of the laser test. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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